anti-Human AES antibody for Western Blotting

Recommended AES Antibody (supplied by: Log in to see )

Antigen
Amino-terminal Enhancer of Split (AES) Antibodies
  • grg
  • esp1
  • grg5
  • tle5
  • aes-1
  • aes-2
  • AES
  • AES-1
  • AES-2
  • ESP1
  • GRG
  • GRG5
  • TLE5
  • AL024115
  • Esp1
  • Grg
  • Grg-5
  • Grg5
  • Tle5
  • zgc:64216
  • xgrg-5
  • similar to amino-terminal enhancer of split
  • amino-terminal enhancer of split
  • LOC100139632
  • aes
  • AES
  • Aes
Epitope
Middle Region
33
22
14
5
4
3
3
2
2
1
1
1
Reactivity
Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish
91
41
31
9
7
6
4
3
2
1
1
Host
Rabbit
71
14
7
Clonality
Polyclonal
Conjugate
This AES antibody is un-conjugated
4
4
4
4
4
4
1
Application
Western Blotting (WB)
79
41
12
8
7
6
3
3
2
1
1
1
1
Options
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Catalog No. ABIN2779565
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN658036 WB Rabbit Ig AA 156-184, C-Term Log in to see Polyclonal 3
3.3487515 ABIN2774824 WB Rabbit C-Term Log in to see Polyclonal 1
3.3487515 ABIN393219 WB Mouse IgG1 kappa AA 2-111 Log in to see 1E1 5
3.3487515 ABIN2779564 WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN2780218 WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN2477291 IHC (fro) WB Rabbit IgG C-Term Log in to see Polyclonal 3
3.3487515 ABIN2464471 ELISA WB Goat N-Term Log in to see Polyclonal
3.3487515 ABIN2461366 ELISA WB Rabbit Log in to see Polyclonal
3.3487515 ABIN3197865 IC IF WB Rabbit Log in to see Polyclonal
3.3487515 ABIN2736244 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN629632 WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN513162 WB Mouse AA 1-264, full length Log in to see Polyclonal
3.3487515 ABIN2560963 ELISA WB Goat IgG N-Term Log in to see Polyclonal
3.3487515 ABIN927369 WB Rabbit Middle Region Log in to see Polyclonal
3.3487515 ABIN920878 WB Rabbit Log in to see Polyclonal
3.3487515 ABIN1030224 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN1031216 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN2565318 WB Mouse AA 1-264, full length Log in to see Polyclonal
1 ABIN302780 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN5554512 EIA IF WB Rabbit IgG C-Term, N-Term Log in to see Polyclonal

Top referenced anti-AES antibody for Western Blotting

Similar anti-AES Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Zebrafish Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 80 Antibodies 38 Antibodies 29 Antibodies 10 Antibodies 3 Antibodies 7 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 7 Antibodies 4 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 4 Antibodies 4 Antibodies

General

Antigen Amino-terminal Enhancer of Split (AES) Antibodies
Epitope Middle Region
(33), (22), (14), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Zebrafish
(91), (41), (31), (9), (7), (6), (4), (3), (2), (1), (1)
Host Rabbit
(71), (14), (7)
Clonality
Polyclonal
Conjugate This AES antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (1)
Application Western Blotting (WB)
(79), (41), (12), (8), (7), (6), (3), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AES Antibody

Target Details AES Application Details Handling ProductDetails: References for anti-AES antibody (ABIN2779565) Images
Blocking Peptide Blocking peptide for this product available: ABIN972974
Sequence HKQAEIVKRL NGICAQVLPY LSQEHQQQVL GAIERAKQVT APELNSIIRQ
Predicted Reactivity Cow: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against AES. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human AES

Target Details AES

Product Details anti-AES Antibody Application Details Handling ProductDetails: References for anti-AES antibody (ABIN2779565) Images back to top
Antigen
Alternative Name AES (AES Antibody Abstract)
Background AES is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes.The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: AES-1, AES-2, ESP1, GRG, GRG5, TLE5
Protein Interaction Partner: MFAP1, MBNL1, SMAD3, LMO2, LMO1, KRT31, DDX6, CRX, BYSL, BCL6, AES, AEN, CARD9, PARVG, LSM2, HOMEZ, MAGOHB, RNF31, VPS37C, C1orf109, OSGIN1, MED4, PRPF31, GORASP2, KANK2, LDOC1, MTUS2, DIP2A, NCDN, BAHD1, IKZF3, U2AF2, RBPMS, FRS3, NEK6, CALCOCO2, ABI2, T
Protein Size: 264
Molecular Weight 29 kDa
Gene ID 166
NCBI Accession NM_198969, NP_945320
UniProt Q14CJ1
Pathways WNT Signaling

Application Details

Product Details anti-AES Antibody Target Details AES Handling ProductDetails: References for anti-AES antibody (ABIN2779565) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 264 AA

Restrictions For Research Use only

Handling

Product Details anti-AES Antibody Target Details AES Application Details ProductDetails: References for anti-AES antibody (ABIN2779565) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-AES antibody (ABIN2779565)

Product Details anti-AES Antibody Target Details AES Application Details Handling Images back to top
Background publications

Lim, Hao, Shaw, Patel, Szabó, Rual, Fisk, Li, Smolyar, Hill, Barabási, Vidal, Zoghbi: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006

Images

Product Details anti-AES Antibody Target Details AES Application Details Handling ProductDetails: References for anti-AES antibody (ABIN2779565) back to top
Supplier Images
Western Blotting (WB) image for anti-AES antibody (Amino-terminal Enhancer of Split) (Middle Region) (ABIN2779565) WB Suggested Anti-AES Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1...