anti-Mouse (Murine) AES antibody for Enzyme Immunoassay

Recommended AES Antibody (supplied by: Log in to see )

Antigen
Amino-terminal Enhancer of Split (AES) Antibodies
  • grg
  • esp1
  • grg5
  • tle5
  • aes-1
  • aes-2
  • AES
  • AES-1
  • AES-2
  • ESP1
  • GRG
  • GRG5
  • TLE5
  • AL024115
  • Esp1
  • Grg
  • Grg-5
  • Grg5
  • Tle5
  • zgc:64216
  • xgrg-5
  • similar to amino-terminal enhancer of split
  • amino-terminal enhancer of split
  • LOC100139632
  • aes
  • AES
  • Aes
Epitope
C-Term, N-Term
32
21
14
5
4
3
3
2
2
2
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
90
41
31
10
7
7
4
3
3
1
1
Host
Rabbit
71
13
7
Clonality
Polyclonal
Conjugate
This AES antibody is un-conjugated
4
4
4
4
4
4
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
78
40
12
7
7
6
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN5554512
$ 375.38
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5554513 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal

Similar anti-AES Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 9 Antibodies 13 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 12 Antibodies
Immunofluorescence (IF) 6 Antibodies 8 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Western Blotting (WB) 29 Antibodies
Agonist (Agon) 1 Antibodies

General

Antigen Amino-terminal Enhancer of Split (AES) Antibodies
Epitope C-Term, N-Term
(32), (21), (14), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(90), (41), (31), (10), (7), (7), (4), (3), (3), (1), (1)
Host Rabbit
(71), (13), (7)
Clonality
Polyclonal
Conjugate This AES antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(78), (40), (12), (7), (7), (6), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-AES Antibody

Target Details AES Application Details Handling Images
Specificity AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.
Purification Affinity chromatography purified via peptide column
Immunogen Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide
Isotype IgG

Target Details AES

Product Details anti-AES Antibody Application Details Handling Images back to top
Antigen
Alternative Name AES / ESP1 (AES Antibody Abstract)
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).Synonyms: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM
Gene ID 166
NCBI Accession NP_001121
UniProt Q08117
Pathways WNT Signaling

Application Details

Product Details anti-AES Antibody Target Details AES Handling Images back to top
Application Notes ELISA. Western Blot: AES antibody can be used for the detection of AES at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AES Antibody Target Details AES Application Details Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.