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anti-Rat (Rattus) AES antibody for ELISA

Recommended AES Antibody (supplied by: Log in to see )

Antigen
Amino-terminal Enhancer of Split (AES) Antibodies
  • AES
  • AES-1
  • aes-1
  • AES-2
  • aes-2
  • AL024115
  • esp1
  • Esp1
  • ESP1
  • Grg
  • GRG
  • grg
  • Grg-5
  • Grg5
  • GRG5
  • grg5
  • tle5
  • Tle5
  • TLE5
  • xgrg-5
  • zgc:64216
Epitope
C-Term
43
24
15
4
3
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
91
34
22
3
3
2
1
1
Host
Rabbit
75
12
7
Clonality
Polyclonal
Conjugate
This AES antibody is un-conjugated
4
4
4
4
4
4
1
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
81
41
12
9
6
5
3
3
2
1
1
1
Supplier
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Catalog No. ABIN1001757
$ 455.07
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012117105 ABIN2461366 ELISA WB Rabbit Log in to see Polyclonal
0.0012117105 ABIN2155084 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012117105 ABIN1030224 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012117105 ABIN2429153 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN2422571 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012117105 ABIN1887085 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012117105 ABIN1583933 ICC ELISA WB Rabbit Log in to see Polyclonal
0.0012117105 ABIN2560963 ELISA WB Goat IgG N-Term Log in to see Polyclonal

Top referenced anti-AES antibody for ELISA

Similar anti-AES Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 9 Antibodies 14 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 11 Antibodies
Immunofluorescence (IF) 7 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 20 Antibodies
Agonist (Agon) 1 Antibodies

General

Antigen Amino-terminal Enhancer of Split (AES) Antibodies
Epitope C-Term
(43), (24), (15), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(91), (34), (22), (3), (3), (2), (1), (1)
Host Rabbit
(75), (12), (7)
Clonality
Polyclonal
Conjugate This AES antibody is un-conjugated
(4), (4), (4), (4), (4), (4), (1)
Application Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(81), (41), (12), (9), (6), (5), (3), (3), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AES Antibody

Target Details AES Application Details Handling References for anti-AES Antibody (ABIN1001757) Images
Blocking Peptide Blocking peptide for this product available: ABIN1003729
Purification Affinity chromatography purified via peptide column.
Immunogen Rabbit polyclol AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES (Genbank accession No. NP_945320).
Isotype IgG

Target Details AES

Product Details anti-AES Antibody Application Details Handling References for anti-AES Antibody (ABIN1001757) Images back to top
Antigen
Alternative Name Amino Terminal Enhancer of Split (AES Antibody Abstract)
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Pathways WNT Signaling

Application Details

Product Details anti-AES Antibody Target Details AES Handling References for anti-AES Antibody (ABIN1001757) Images back to top
Application Notes AES antibody can be used for the detection of AES by Western blot at 1 - 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only

Handling

Product Details anti-AES Antibody Target Details AES Application Details References for anti-AES Antibody (ABIN1001757) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-AES Antibody (ABIN1001757)

Product Details anti-AES Antibody Target Details AES Application Details Handling Images back to top
Product cited in:

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004

Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000

Background publications

Jan, Matter, Pai, Chen, Pilch, Komatsu, Ong, Fukuda, Ruoslahti: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004

Miyasaka, Choudhury, Hou, Li: "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein." in: European journal of biochemistry / FEBS, Vol. 216, Issue 1, pp. 343-52, 1993