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|Application / Reactivity||Human||Chimpanzee||Horse (Equine)||Monkey||Mouse (Murine)|
|Western Blotting (WB)||36 Antibodies||1 Antibodies||2 Antibodies||2 Antibodies||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies||1 Antibodies||3 Antibodies||3 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Flow Cytometry (FACS)||9 Antibodies|
|Antigen||G Protein-Coupled Bile Acid Receptor 1 (GPBAR1) Antibodies|
|Epitope||AA 302-322 Alternatives|
|Reactivity||Chimpanzee, Horse (Equine), Human, Monkey, Mouse (Murine) Alternatives|
|Conjugate||This GPBAR1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-GPBAR1 AntibodyTarget Details GPBAR1 Application Details Handling Images
|Specificity||Amino acids 302-322 of Human BG37/TGR5|
Species reactivity (expected):Chimpanzee, Horse, Monkey and Mouse.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide (KLH conjugated) corresponding to a portion of the amino acids 302-322 of Human BG37/TGR5.|
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|Alternative Name||GPBAR1 / TGR5 (GPBAR1 Antibody Abstract)|
|Background||GPBAR1/TGR5 is a member of the G protein-coupled receptor family (GPCRs, or GPRs) which contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR TGR5 is a receptor for bile acids. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. It may be involved in the suppression of macrophage functions by bile acids.Synonyms: BG37, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, M-BAR, Membrane-type receptor for bile acids|
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Western Blot: 2-4 μg/mL. Immunohistochemistry on Paraffin Sections: 10 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen. The stained slides were evaluated by apathologist to confirm staining specificity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS containing 0.05 % Sodium Azide as preservative and 0.2 % Gelatin as stabilizer.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
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