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Human IGFBP4 ELISA Kit

Recommended IGFBP4 Product (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kits
  • IGFBP4
  • igfbp4
  • BP-4
  • HT29-IGFBP
  • IBP4
  • IGFBP-4
  • AI875747
  • Deb2
  • IGF-BP4
  • IBP-4
  • insulin-like growth factor binding protein 4
  • IGFBP4
  • igfbp4
  • LOC100194498
  • LOC100225608
  • LOC100271886
  • Igfbp4
  • LOC100337926
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
16
10
10
7
7
4
3
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
0.25-100 ng/mL
Minimum Detection Limit
0.25 ng/mL
Application
ELISA
Options
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Catalog No. ABIN625000
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
12.868749 ABIN841738 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 31.25-2000 pg/mL 31.25 pg/mL Log in to see
12.868749 ABIN414871 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
12.868749 ABIN2859270 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA) 500-32000 pg/mL 500 pg/mL Log in to see
12.868749 ABIN1115525 Sandwich ELISA Serum, Plasma, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
12.868749 ABIN1028911 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN510006 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
1 ABIN3073434 Sandwich ELISA Plasma, Serum 4.75-165 ng/mL 4.75 ng/mL Log in to see
1 ABIN3073435 Sandwich ELISA Plasma, Serum 9-255 ng/mL 9 ng/mL Log in to see
1 ABIN2648687 Biological Fluids Log in to see
1 ABIN2534713 Serum, Plasma, Biological Fluids 0.6-40 ng/mL 0.6 ng/mL Log in to see
1 ABIN2535078 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Culture Supernatant, Biological Fluids 3.13-200 ng/mL 3.13 ng/mL Log in to see
1 ABIN4970208 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 3.125-200 ng/mL 3.125 ng/mL Log in to see
1 ABIN455464 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN2948566 3.125-200 ng/mL 3.125 ng/mL Log in to see

Top referenced IGFBP4 ELISA Kit for ELISA

Similar IGFBP4 ELISA Kits

Application / Reactivity Human
ELISA 15 ELISA Kits

General

Antigen Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(16), (10), (10), (7), (7), (4), (3), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 0.25-100 ng/mL
Minimum Detection Limit 0.25 ng/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details IGFBP4 ELISA Kit

Target details Application Details Handling References for IGFBP4 Kit (ABIN625000) Images
Purpose Human IGFBP-4 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GCSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity 250 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details IGFBP4 ELISA Kit Application Details Handling References for IGFBP4 Kit (ABIN625000) Images back to top
Antigen
Alternative Name IGFBP-4 (IGFBP4 ELISA Kit Abstract)
Background IGF-BPs (Insulin-like growth factor binding proteins) are found in various body fluids such as blood serum, amniotic fluid, and liquor. They are synthesized in the liver and are produced also by various tumor cell lines and cell types. IGFBP-4 is the predominant IGF binding proteins expressed by human osteoblast-like cells. It is identical with a protein known as Colon cancer cell growth inhibitor. IGFBP-4 has been overexpressed in the malignant M12 prostate epithelial cell line to determine the effects on tumor formation and apoptosis. Overexpressing cells show reduced proliferation in response to IGF and inhibited colony growth. The Human IGFBP-4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IGFBP-4 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human IGFBP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IGFBP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 3487
UniProt P22692
Pathways WNT Signaling

Application Details

Product Details IGFBP4 ELISA Kit Target details Handling References for IGFBP4 Kit (ABIN625000) Images back to top
Application Notes Recommended Dilution for serum and plasma samples2 - 10 fold
Plate Pre-coated,Strips (12 x 8)
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) is used for dilution of serum/plasma samples, and Assay Diluent B (Item E) is used for dilution of culture supernatants and urine. 3. Assay Diluent B should be diluted 5-fold with deionized or distilled water. 4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µl Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine) into Item C vial to prepare a 0.2µg/ml standard. Dissolve the powder thoroughly by a gentle mix. Add 250 µl IGFBP-4 standard from the vial of Item C, into a tube with 250 µl Assay Diluent A or 1x Assay Diluent B to prepare a 100.0 ng/ml stock standard solution. Pipette 400 µl Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 ng/ml). 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µl of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) before use. HRP-Streptavidin concentrate should be diluted 30,000-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 2 µl of HRP-Streptavidin concentrate into a tube with 198.0 µl 1x Assay Diluent B to prepare a 100-fold diluted HRP-Streptavidin solution (do not store the diluted solution for next day use). Mix through and then pipette 50 µl of prepared 100-fold diluted solution into a tube with 15 ml 1x Assay Diluent B to prepare a final 30,000 fold diluted HRP-Streptavidin solution.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µl of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking. 3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µl of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µl of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µl of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µl of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Restrictions For Research Use only

Handling

Product Details IGFBP4 ELISA Kit Target details Application Details References for IGFBP4 Kit (ABIN625000) Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

References for IGFBP4 Kit (ABIN625000)

Product Details IGFBP4 ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Liu, Liu, Zhou, Xiao, Cao: "Conditioned medium from chondrocyte/scaffold constructs induced chondrogenic differentiation of bone marrow stromal cells." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 295, Issue 7, pp. 1109-16, 2012

Chelchowska, Maciejewski, Gajewska, Ambroszkiewicz, Laskowska-Klita, Leibschang: "The pregnancy-associated plasma protein A and insulin-like growth factor system in response to cigarette smoking." in: The journal of maternal-fetal & neonatal medicine, Vol. 25, Issue 11, pp. 2377-80, 2012

Images

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Supplier Images
ELISA image for IGFBP4 ELISA Kit (Insulin-Like Growth Factor Binding Protein 4) (ABIN625000) Insulin-Like Growth Factor Binding Protein 4 (IGFBP4) ELISA Kit