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anti-Human IGFBP5 antibody for Enzyme Immunoassay

Recommended IGFBP5 Antibody (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) Antibodies
  • AI256729
  • AW208790
  • IBP-5
  • IBP5
  • ibp5
  • IGF-BP5
  • IGFBP-5
  • IGFBP-5P
  • IGFBP5
  • igfbp5
  • xIGFBP-5
Reactivity
Human
88
37
33
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
84
17
5
3
Clonality
Polyclonal
Conjugate
This IGFBP5 antibody is un-conjugated
14
7
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
77
52
31
12
11
6
5
2
1
1
1
Supplier
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Catalog No. ABIN181656
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008806384 ABIN181653 EIA WB Biotin Rabbit Log in to see Polyclonal

Similar anti-IGFBP5 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 37 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 66 Antibodies

General

Antigen Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) Antibodies
Reactivity Human
(88), (37), (33), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(84), (17), (5), (3)
Clonality
Polyclonal
Conjugate This IGFBP5 antibody is un-conjugated
(14), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(77), (52), (31), (12), (11), (6), (5), (2), (1), (1), (1)
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Product Details anti-IGFBP5 Antibody

Target Details IGFBP5 Application Details Handling Images
Specificity This antibody detects Insulin-like Growth Factor Binding Protein 5.
Purification Immunoaffinity Chromatography
Immunogen Highly pure (> 98 %) recombinant human IGF-BP5

Target Details IGFBP5

Product Details anti-IGFBP5 Antibody Application Details Handling Images back to top
Antigen
Alternative Name IGFBP5 (IGFBP5 Antibody Abstract)
Background IGFBP5 is one of a family of proteins that bind to insulin-like growth factors (IGFs). IGFBP5 is a multifunctional protein which acts not only as a traditional binding protein but also functions as a growth factor independent of IGFs to stimulate bone formation. Currently there are seven named IGF-BPs that form high affinity complexes with both IGFI and IGFII. IGFBP5 is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGFI on smooth muscle cells, fibroblasts or osteoblasts. Data shows that IGFBP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGFBP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole-body growth inhibition and retarded muscle development.Synonyms: IBP-5, IBP5, IGF-binding protein 5, IGFBP-5, Insulin-like growth factor-binding protein 5
Gene ID 3488
NCBI Accession NP_000590
UniProt P24593
Research Area Signaling, Diabetes, Metabolism, Growth Factors
Pathways WNT Signaling

Application Details

Product Details anti-IGFBP5 Antibody Target Details IGFBP5 Handling Images back to top
Application Notes Indirect ELISA: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentration of0.5-2.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Sandwich ELISA: To detect hIGF-BP5 (using 100 μL/well antibody solution) a concentrationof 0.5-2.0 μg/mL of this antibody is required. In conjunction with Biotinylated Anti-HumanIGF-BP5 (ABIN181653 or ABIN181654) as a detection antibody, it allows the detection ofat least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western blot: To detect hIGF-BP5 this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit forrecombinant hIGF-BP5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-IGFBP5 Antibody Target Details IGFBP5 Application Details Images back to top
Reconstitution Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Expiry Date 6 months

Images

Product Details anti-IGFBP5 Antibody Target Details IGFBP5 Application Details Handling back to top
Supplier Images
Enzyme Immunoassay (EIA) image for anti-IGFBP5 antibody (Insulin-Like Growth Factor Binding Protein 5) (ABIN181656) Sandwich ELISA using IGFBP5 Antibody Cat.-No AP01139PU
Immunofluorescence (IF) image for anti-IGFBP5 antibody (Insulin-Like Growth Factor Binding Protein 5) (ABIN181656) This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated a...
Western Blotting (WB) image for anti-IGFBP5 antibody (Insulin-Like Growth Factor Binding Protein 5) (ABIN181656) Western Blot (Reduced) using IGFBP5 Antibody Cat.-No AP01139PU
Western Blotting (WB) image for anti-IGFBP5 antibody (Insulin-Like Growth Factor Binding Protein 5) (ABIN181656) Western Blot (Unreduced) using IGFBP5 Antibody Cat.-No AP01139PU