Rat (Rattus) IGFBP5 ELISA Kit

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Antigen
Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kits
  • igfbp5
  • ibp5
  • IBP-5
  • IGFBP-5
  • xIGFBP-5
  • IGFBP5
  • IBP5
  • AI256729
  • AW208790
  • IGFBP-5P
  • IGF-BP5
  • insulin-like growth factor binding protein 5a
  • insulin-like growth factor binding protein 5
  • insulin-like growth factor-binding protein 5
  • igfbp5a
  • igfbp5
  • IGFBP5
  • LOC100194500
  • Igfbp5
  • LOC100348897
  • IGFBP-5
Epitope
AA 20-271
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
15
14
14
7
4
2
2
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
156-10.000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
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Catalog No. ABIN1889438
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.76768 ABIN416462 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
9.76768 ABIN2703127 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 25-7.000 pg/mL 25 pg/mL Log in to see
9.76768 ABIN1115527 Sandwich ELISA Serum, Plasma, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN3032870 Competition ELISA Cell Culture Supernatant, Plasma, Serum 28.7-7000 pg/mL 28.7 pg/mL Log in to see
1 ABIN366466 Sandwich ELISA Serum, Plasma, Tissue Homogenate 93.75-6000 pg/mL 93.75 pg/mL Log in to see
1 ABIN627202 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 50-1000 pg/mL 50 pg/mL Log in to see
1 ABIN1570550 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN5523444 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN4885924 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Log in to see
1 ABIN2748825 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN1138189 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN4970211 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
1 ABIN2535083 Sandwich ELISA Serum, Plasma, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
1 ABIN2948530 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN5073054 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

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General

Antigen Insulin-Like Growth Factor Binding Protein 5 (IGFBP5) ELISA Kits
Epitope AA 20-271
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(15), (14), (14), (7), (4), (2), (2), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 156-10.000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details IGFBP5 ELISA Kit

Target details Application Details Handling ProductDetails: References for IGFBP5 Kit (ABIN1889438) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGFBP5
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: L20-E271
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: L20-E271

Target details

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Antigen
Alternative Name IGFBP5 (IGFBP5 ELISA Kit Abstract)
Background

Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

Synonyms: Insulin-like growth factor-binding protein 5,IBP-5,IGF-binding protein 5,IGFBP-5,Igfbp5,Igfbp-5,

Full Gene Name: Insulin-like growth factor-binding protein 5

Cellular Localisation: Secreted.
Gene ID 25285
UniProt P24594
Research Area Signaling, Diabetes, Metabolism, Growth Factors
Pathways WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding

Application Details

Product Details IGFBP5 ELISA Kit Target details Handling ProductDetails: References for IGFBP5 Kit (ABIN1889438) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Contains 1 IGFBP N-terminal domain.

Tissue Specificity: Mostly in kidney.

Plate Pre-coated
Protocol rat IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGFBP5 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat IGFBP5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IGFBP5 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.32, Standard deviation: 0.055, CV(%): 4.2
  • Sample 2: n=16, Mean(ng/ml): 4.14, Standard deviation: 0.162, CV(%): 3.9
  • Sample 3: n=16, Mean(ng/ml): 6.88, Standard deviation: 0.234, CV(%): 3.4,
  • Sample 1: n=24, Mean(ng/ml): 1.45, Standard deviation: 0.096, CV(%): 6.6
  • Sample 2: n=24, Mean(ng/ml): 4.24, Standard deviation: 0.225, CV(%): 5.3
  • Sample 3: n=24, Mean(ng/ml): 7.26, Standard deviation: 0.348, CV(%): 4.8
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

ProductDetails: References for IGFBP5 Kit (ABIN1889438)

Product Details IGFBP5 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Butt, Dickson, McDougall, Baxter: "Insulin-like growth factor-binding protein-5 inhibits the growth of human breast cancer cells in vitro and in vivo." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29676-85, 2003

Allander, Larsson, Ehrenborg, Suwanichkul, Weber, Morris, Bajalica, Kiefer, Luthman, Powell: "Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5." in: The Journal of biological chemistry, Vol. 269, Issue 14, pp. 10891-8, 1994

Images

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Supplier Images
ELISA image for IGFBP5 ELISA Kit (Insulin-Like Growth Factor Binding Protein 5) (ABIN1889438) Rat IGFBP5 PicoKine ELISA Kit standard curve