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IGFBP5 ELISA Kit

IGFBP5 Reactivity: Rat AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN1889438
  • Target See all IGFBP5 ELISA Kits
    IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
    Binding Specificity
    AA 20-271
    Reactivity
    • 8
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGFBP5
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: L20-E271
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: L20-E271
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Tissue Specificity: Mostly in kidney.

    Plate
    Pre-coated
    Protocol
    rat IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IGFBP5 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat IGFBP5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat IGFBP5 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.32, Standard deviation: 0.055, CV(%): 4.2
    • Sample 2: n=16, Mean(ng/ml): 4.14, Standard deviation: 0.162, CV(%): 3.9
    • Sample 3: n=16, Mean(ng/ml): 6.88, Standard deviation: 0.234, CV(%): 3.4,
    • Sample 1: n=24, Mean(ng/ml): 1.45, Standard deviation: 0.096, CV(%): 6.6
    • Sample 2: n=24, Mean(ng/ml): 4.24, Standard deviation: 0.225, CV(%): 5.3
    • Sample 3: n=24, Mean(ng/ml): 7.26, Standard deviation: 0.348, CV(%): 4.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all IGFBP5 ELISA Kits
    IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
    Alternative Name
    IGFBP5 (IGFBP5 Products)
    Synonyms
    IBP5 ELISA Kit, AI256729 ELISA Kit, AW208790 ELISA Kit, IGFBP-5 ELISA Kit, IGFBP-5P ELISA Kit, IGF-BP5 ELISA Kit, igfbp5 ELISA Kit, ibp5 ELISA Kit, IBP-5 ELISA Kit, xIGFBP-5 ELISA Kit, IGFBP5 ELISA Kit, igfbp5b ELISA Kit, zgc:165472 ELISA Kit, insulin like growth factor binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5 ELISA Kit, insulin like growth factor binding protein 5 L homeolog ELISA Kit, insulin-like growth factor binding protein 5b ELISA Kit, insulin-like growth factor-binding protein 5 ELISA Kit, insulin-like growth factor binding protein 5a ELISA Kit, IGFBP5 ELISA Kit, Igfbp5 ELISA Kit, igfbp5.L ELISA Kit, igfbp5b ELISA Kit, igfbp5 ELISA Kit, LOC100194500 ELISA Kit, igfbp5a ELISA Kit
    Background

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

    Synonyms: Insulin-like growth factor-binding protein 5,IBP-5,IGF-binding protein 5,IGFBP-5,Igfbp5,Igfbp-5,

    Full Gene Name: Insulin-like growth factor-binding protein 5

    Cellular Localisation: Secreted.
    Gene ID
    25285
    UniProt
    P24594
    Pathways
    WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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