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Human IGFBP6 ELISA Kit

Recommended IGFBP6 Product (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kits
  • IBP6
  • IGF-BP6
  • IGFBP-6
  • igfbp6
  • IGFBP6
  • RBP6A
Epitope
AA 25-240
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
12
11
10
7
5
3
3
2
2
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1672866
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30647367 ABIN415186 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.62-40 ng/mL 0.62 ng/mL Log in to see
0.30647367 ABIN1115528 Sandwich ELISA Serum, Plasma, Biological Fluids 0.16-10 ng/mL 0.16 ng/mL Log in to see
0.30647367 ABIN1570552 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.813-500 ng/mL 7.813 ng/mL Log in to see
0.30647367 ABIN2708250 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN841743 Sandwich ELISA Serum, Plasma, Tissue Homogenate 20-0.31 ng/mL 20 ng/mL Log in to see
0.0012153484 ABIN510008 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 25-500 ng/mL 25 ng/mL Log in to see
0.0012153484 ABIN625002 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 1
0.0012153484 ABIN2042508 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012153484 ABIN1138191 78-5000 pg/mL 78 pg/mL Log in to see
0.0012153484 ABIN2088081 Sandwich ELISA Log in to see
0.0012153484 ABIN2948569 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012153484 ABIN2948531 0.625-40 ng/mL 0.625 ng/mL Log in to see

Top referenced IGFBP6 ELISA Kit for ELISA

Similar IGFBP6 ELISA Kits

Application / Reactivity Human
ELISA 13 ELISA Kits

General

Antigen Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kits
Epitope AA 25-240
Reactivity Human
Kits with alternative reactivity to:
(12), (11), (10), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details IGFBP6 ELISA Kit

Target details Application Details Handling References for IGFBP6 Kit (ABIN1672866) Images
Purpose For quantitative detection of human IGFBP6 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human IGFBP6
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human IGFBP6 antibod - 1
  • Lyophilized recombinant human IGFBP6 standar - 10ng/tubex2
  • Biotinylated anti- human IGFBP6 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A25-G240

Target details

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Antigen
Alternative Name IGFBP6 (IGFBP6 ELISA Kit Abstract)
Background Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.
Gene ID 3489
UniProt P24592
Research Area Signaling, Diabetes, Metabolism, Growth Factors
Pathways WNT Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human IGFBP6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP6 has been precoated onto 96-well plates. Standards(NSO, A25-G240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP6 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP6 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 551, Standard deviation: 21.5, CV(%): 3.9
  • Sample 2: n=16, Mean(pg/ml): 1557, Standard deviation: 63.84, CV(%): 4.1
  • Sample 3: n=16, Mean(pg/ml): 2736, Standard deviation: 128.6, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 489, Standard deviation: 27.87, CV(%): 5.7
  • Sample 2: n=24, Mean(pg/ml): 1635, Standard deviation: 101.37, CV(%): 6.2
  • Sample 3: n=24, Mean(pg/ml): 2878, Standard deviation: 184.2, CV(%): 6.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IGFBP6 Kit (ABIN1672866)

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Background publications

Kiefer, Ioh, Bauer, Zapf: "Molecular cloning of a new human insulin-like growth factor binding protein." in: Biochemical and biophysical research communications, Vol. 176, Issue 1, pp. 219-25, 1991

Images

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Supplier Images
ELISA image for IGFBP6 ELISA Kit (Insulin-Like Growth Factor Binding Protein 6) (ABIN1672866) Human IGFBP6 PicoKine ELISA Kit standard curve