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Human IGFBP6 ELISA Kit

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Antigen
Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kits
  • IGFBP-6
  • IGFBP6
  • igfbp6
  • IBP6
  • IGF-BP6
  • RBP6A
  • insulin-like growth factor binding protein 6
  • insulin-like growth factor binding protein-6
  • IGFBP-6
  • IGFBP6
  • igfbp6
  • Igfbp6
Epitope
AA 25-240
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
12
12
7
4
3
2
2
2
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
Options
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Catalog No. ABIN1672866
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
11.361965 ABIN625002 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 150-60000 pg/mL 150 pg/mL Log in to see 2
11.361965 ABIN415186 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
11.361965 ABIN1570552 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.812-500 ng/mL 7.812 ng/mL Log in to see
11.361965 ABIN1115528 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
11.361965 ABIN2708250 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN841743 Sandwich ELISA Serum, Plasma, Tissue Homogenate 20-0.31 ng/mL 20 ng/mL Log in to see
1 ABIN4993104 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.082-60 ng/mL 0.082 ng/mL Log in to see
1 ABIN510008 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 25-500 ng/mL 25 ng/mL Log in to see
1 ABIN5523445 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4885252 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum Log in to see
1 ABIN2042508 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN5499319 Sandwich ELISA Cell Lysate 9.38-600 pg/mL 9.38 pg/mL Log in to see
1 ABIN1138191 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN4970217 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 7.812-500 ng/mL 7.812 ng/mL Log in to see
1 ABIN2948569 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN2948531 0.625-40 ng/mL 0.625 ng/mL Log in to see
1 ABIN5069123 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

Top referenced IGFBP6 ELISA Kit for ELISA

Similar IGFBP6 ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Insulin-Like Growth Factor Binding Protein 6 (IGFBP6) ELISA Kits
Epitope AA 25-240
Reactivity Human
Kits with alternative reactivity to:
(17), (12), (12), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details IGFBP6 ELISA Kit

Target details Application Details Handling References for IGFBP6 Kit (ABIN1672866) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP6
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: A25-G240
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A25-G240

Target details

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Antigen
Alternative Name IGFBP6 (IGFBP6 ELISA Kit Abstract)
Background

Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Background: Insulin-like growth factor-binding protein 6(IGFBP6) is a protein that in humans is encoded by the IGFBP6 gene. The IGFBP6 gene is mapped to chromosome 12. In all, it has contained 216 amino acid protein with a calculated molecular mass of 22,847, The human keratinocyte cell line HaCat secretes IGFBP6 as an autocrine growth inhibitor. Recombinant IGFBP6 was also shown to inhibit growth of HaCat cells and other keratinocyte cell lines.

Synonyms: Insulin-like growth factor-binding protein 6,IBP-6,IGF-binding protein 6,IGFBP-6,IGFBP6,IBP6,

Full Gene Name: Insulin-like growth factor-binding protein 6

Cellular Localisation: Secreted.
Gene ID 3489
UniProt P24592
Research Area Signaling, Diabetes, Metabolism, Growth Factors
Pathways WNT Signaling, Myometrial Relaxation and Contraction

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Contains 1 IGFBP N-terminal domain.

Plate Pre-coated,Strips (12 x 8)
Protocol human IGFBP6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP6 has been precoated onto 96-well plates. Standards(NSO, A25-G240) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP6 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGFBP6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGFBP6 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 551, Standard deviation: 21.5, CV(%): 3.9
  • Sample 2: n=16, Mean(pg/ml): 1557, Standard deviation: 63.84, CV(%): 4.1
  • Sample 3: n=16, Mean(pg/ml): 2736, Standard deviation: 128.6, CV(%): 4.7,
  • Sample 1: n=24, Mean(pg/ml): 489, Standard deviation: 27.87, CV(%): 5.7
  • Sample 2: n=24, Mean(pg/ml): 1635, Standard deviation: 101.37, CV(%): 6.2
  • Sample 3: n=24, Mean(pg/ml): 2878, Standard deviation: 184.2, CV(%): 6.4
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for IGFBP6 Kit (ABIN1672866)

Product Details IGFBP6 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Kiefer, Ioh, Bauer, Zapf: "Molecular cloning of a new human insulin-like growth factor binding protein." in: Biochemical and biophysical research communications, Vol. 176, Issue 1, pp. 219-25, 1991

Images

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Supplier Images
ELISA image for IGFBP6 ELISA Kit (Insulin-Like Growth Factor Binding Protein 6) (ABIN1672866) Human IGFBP6 PicoKine ELISA Kit standard curve