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anti-Human LEF1 antibody for RNA Interference

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Epitope
AA 1-399, full length
21
16
16
6
6
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
117
33
25
7
4
4
3
2
1
1
1
1
1
Host
Mouse
53
44
16
5
Clonality
Monoclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
RNA Interference (RNAi), ELISA, Western Blotting (WB)
107
61
19
11
5
3
3
2
2
2
1
1
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Catalog No. ABIN565556
$ 430.00
Plus shipping costs $45.00 and $21.00 dry ice

Top referenced anti-LEF1 antibody for RNA Interference

Similar anti-LEF1 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 63 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 3 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 109 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope AA 1-399, full length
(21), (16), (16), (6), (6), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(117), (33), (25), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(53), (44), (16), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application RNA Interference (RNAi), ELISA, Western Blotting (WB)
(107), (61), (19), (11), (5), (3), (3), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN565556) Images
Cross-Reactivity Human
Immunogen LEF1 (AAH50632, 1 a.a. ~ 399 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS PGGVGQITPP LGWQGQPVYP ITGGFRQPYP SSLSVDTSMS RFSHHMIPGP PGPHTTGIPH PAIVTPQVKQ EHPHTDSDLM HVKPQHEQRK EQEPKRPHIK KPLNAFMLYM KEMRANVVAE CTLKESAAIN QILGRRWHAL SREEQAKYYE LARKERQLHM QLYPGWSARD NYGKKKKRKR EKLQESASGT GPRMTAAYI
Clone 3H5
Isotype IgG1 kappa

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 Antibody (ABIN565556) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Full Gene Name: lymphoid enhancer-binding factor 1
Synonyms: DKFZp586H0919,TCF1ALPHA
Gene ID 51176
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 Antibody (ABIN565556) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Product Quality tested by: Antibody Reactive Against Recombinant Protein.

Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 Antibody (ABIN565556) Images back to top
Buffer In 1x PBS, pH 7.4
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-LEF1 Antibody (ABIN565556)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Product cited in:

Jamieson, Sharma, Henderson: "Regulation of ?-catenin nuclear dynamics by GSK-3? involves a LEF-1 positive feedback loop." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 8, pp. 983-99, 2011

Hebenstreit, Giaisi, Treiber, Zhang, Mi, Horejs-Hoeck, Andersen, Krammer, Duschl, Li-Weber: "LEF-1 negatively controls interleukin-4 expression through a proximal promoter regulatory element." in: The Journal of biological chemistry, Vol. 283, Issue 33, pp. 22490-7, 2008

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 Antibody (ABIN565556) back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 1-399) (ABIN565556) LEF1 monoclonal antibody (M01), clone 3H5 Western Blot analysis of LEF1 expression in...
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 1-399) (ABIN565556) Western blot analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 V...
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 1-399) (ABIN565556) LEF1 monoclonal antibody (M01), clone 3H5. Western Blot analysis of LEF1 expression i...
ELISA image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 1-399) (ABIN565556) Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a captur...
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 1-399) (ABIN565556) Western Blot analysis of LEF1 expression in transfected 293T cell line by LEF1 monocl...
 image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (AA 1-399) (ABIN565556) Western Blot detection against Immunogen (69.63 KDa) .