anti-Rat (Rattus) LEF1 antibody for ELISA

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Epitope
Internal Region
15
13
12
9
8
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
128
41
31
11
7
7
5
5
4
4
4
4
3
2
1
1
Host
Goat
57
52
15
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
115
66
22
17
10
7
5
3
2
2
2
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN185607
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN297259 ELISA WB Goat AA 257-270 Log in to see Polyclonal
1 ABIN188546 ELISA WB Goat Log in to see Polyclonal 2
1 ABIN1531685 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
1 ABIN3182271 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
1 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2465242 ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN1584697 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2883996 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
1 ABIN2563660 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN790949 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1088252 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1088251 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2428345 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2421791 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2420659 ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN2687988 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1577619 ELISA WB Rabbit pSer42 Log in to see Polyclonal
1 ABIN802662 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN1856841 ELISA WB Rabbit Log in to see Polyclonal

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope Internal Region
(15), (13), (12), (9), (8), (6), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(128), (41), (31), (11), (7), (7), (5), (5), (4), (4), (4), (4), (3), (2), (1), (1)
Host Goat
(57), (52), (15), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Method Type Peptide ELISA
Application ELISA, Western Blotting (WB)
(115), (66), (22), (17), (10), (7), (5), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling ProductDetails: References for anti-LEF1 antibody (ABIN185607) Images
Blocking Peptide Blocking peptide for this product available: ABIN369644
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling ProductDetails: References for anti-LEF1 antibody (ABIN185607) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Synonyms: LEF1, lymphoid enhancer-binding factor 1, HGNC:6551, DKFZp586H0919, TCF1ALPHA, lymphoid enhancer binding factor-1
Gene ID 51176, 16842, 161452
NCBI Accession NP_057353
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling ProductDetails: References for anti-LEF1 antibody (ABIN185607) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48 kDa band observed in lysates of cell lines Daudi and Jurkat (calculated MW of 44.2 kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yasumoto et al , EMBO J. 2002 Jun 3,21(11):2703-14., PMID: 12032083). The same band was also observed in Mouse and Rat Spleen lysates. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details ProductDetails: References for anti-LEF1 antibody (ABIN185607) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

ProductDetails: References for anti-LEF1 antibody (ABIN185607)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Radtke, Clevers: "Self-renewal and cancer of the gut: two sides of a coin." in: Science (New York, N.Y.), Vol. 307, Issue 5717, pp. 1904-9, 2005

Waterman: "Lymphoid enhancer factor/T cell factor expression in colorectal cancer." in: Cancer metastasis reviews, Vol. 23, Issue 1-2, pp. 41-52, 2004

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling ProductDetails: References for anti-LEF1 antibody (ABIN185607) back to top
Supplier Images
 image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody (ABIN185607) ABIN185607 (1µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Prim...