anti-Rat (Rattus) LEF1 antibody for Western Blotting

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • LEF1
  • 3000002B05
  • AI451430
  • Lef-1
  • LEF-1
  • TCF10
  • TCF1ALPHA
  • TCF7L3
  • lymphoid enhancer-binding factor 1
  • lymphocyte enhancer binding factor 1
  • lymphoid enhancer binding factor 1
  • lymphoid enhancer factor XLEF-1B
  • LEF1
  • LOC100352691
  • lef1
  • Lef1
Epitope
N-Term
15
13
12
9
8
6
6
6
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
128
40
30
10
6
6
5
4
4
4
4
4
3
2
1
1
Host
Rabbit
56
52
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
115
67
22
17
10
7
5
3
2
2
2
1
1
1
1
Options
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Catalog No. ABIN2792635
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2182703 ABIN2779620 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.2182703 ABIN2779621 WB Rabbit Middle Region Log in to see Polyclonal
3.2182703 ABIN735248 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
3.2182703 ABIN1531685 ELISA WB Rabbit IgG AA 8-57, pSer42 Log in to see Polyclonal
3.2182703 ABIN188546 ELISA WB Goat Log in to see Polyclonal 2
3.2182703 ABIN3182271 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
3.2182703 ABIN2460458 IHC ELISA WB Rabbit Log in to see Polyclonal
3.2182703 ABIN2465242 ELISA WB Goat Internal Region Log in to see Polyclonal
3.2182703 ABIN2563660 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1387756 IF (p) IHC (p) WB Rabbit IgG pSer42 Log in to see Polyclonal 1
1 ABIN185607 ELISA WB Goat Internal Region Log in to see Polyclonal 2
1 ABIN202428 IHC (p) WB Rabbit IgG AA 282-331 Log in to see Polyclonal
1 ABIN297259 ELISA WB Goat AA 257-270 Log in to see Polyclonal
1 ABIN5553187 WB Goat Internal Region Log in to see Polyclonal
1 ABIN221435 ICC FACS IF IP WB Rabbit IgG Pro82 Log in to see
1 ABIN1584697 IHC ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2883996 ELISA WB Rabbit IgG pSer42 Log in to see Polyclonal
1 ABIN790949 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1088251 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1088252 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Epitope N-Term
(15), (13), (12), (9), (8), (6), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(128), (40), (30), (10), (6), (6), (5), (4), (4), (4), (4), (4), (3), (2), (1), (1)
Host Rabbit
(56), (52), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(115), (67), (22), (17), (10), (7), (5), (3), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling References for anti-LEF1 antibody (ABIN2792635) Images
Sequence VARQAQTSQE PYHDKAREHP DDGKHPDGGL YNKGPSYSSY SGYIMMPNMN
Cross-Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
Predicted Reactivity Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 85%, Rat: 92%
Characteristics This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human LEF1

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling References for anti-LEF1 antibody (ABIN2792635) Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399
Molecular Weight 44 kDa
Gene ID 51176
NCBI Accession NM_016269, NP_057353
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling References for anti-LEF1 antibody (ABIN2792635) Images back to top
Application Notes WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 399 AA

Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details References for anti-LEF1 antibody (ABIN2792635) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-LEF1 antibody (ABIN2792635)

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling Images back to top
Background publications

Petropoulos, Arseni, Schessl, Stadler, Rawat, Deshpande, Heilmeier, Hiddemann, Quintanilla-Martinez, Bohlander, Feuring-Buske, Buske: "A novel role for Lef-1, a central transcription mediator of Wnt signaling, in leukemogenesis." in: The Journal of experimental medicine, Vol. 205, Issue 3, pp. 515-22, 2008

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling References for anti-LEF1 antibody (ABIN2792635) back to top
Supplier Images
Western Blotting (WB) image for anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (N-Term) antibody (ABIN2792635) WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...