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anti-Zebrafish LEF1 antibody for ELISA

Recommended LEF1 Antibody (supplied by: Log in to see )

Antigen
Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
  • 3000002B05
  • AI451430
  • LEF-1
  • Lef-1
  • LEF1
  • TCF1ALPHA
  • TCF7L3
  • TCF10
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish
117
32
24
6
4
4
3
1
1
1
1
1
1
Host
Rabbit
52
45
16
5
Clonality
Polyclonal
Conjugate
This LEF1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
107
61
19
10
5
3
3
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2460458
$ 343.75
Plus shipping costs $45.00

Similar anti-LEF1 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus) Zebrafish
Western Blotting (WB) 109 Antibodies 7 Antibodies 29 Antibodies 21 Antibodies 2 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies 3 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 3 Antibodies 8 Antibodies 7 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 2 Antibodies 2 Antibodies 1 Antibodies
Immunofluorescence (IF) 20 Antibodies 2 Antibodies

General

Antigen Lymphoid Enhancer-Binding Factor 1 (LEF1) Antibodies
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Zebrafish
(117), (32), (24), (6), (4), (4), (3), (1), (1), (1), (1), (1), (1)
Host Rabbit
(52), (45), (16), (5)
Clonality
Polyclonal
Conjugate This LEF1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(107), (61), (19), (10), (5), (3), (3), (2), (2), (2), (1), (1), (1)
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Product Details anti-LEF1 Antibody

Target Details LEF1 Application Details Handling Images
Purification Antibody is purified by protein A chromatography method.
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.

Target Details LEF1

Product Details anti-LEF1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name LEF1 (LEF1 Antibody Abstract)
Background Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1.
Molecular Weight 44 kDa
Gene ID 51176
NCBI Accession NP_057353
UniProt Q9UJU2
Research Area Transcription Factors, Wnt, Lineage Markers
Pathways WNT Signaling

Application Details

Product Details anti-LEF1 Antibody Target Details LEF1 Handling Images back to top
Application Notes LEF1 antibody can be used for detection of LEF1 by ELISA at 1:62500. LEF1 antibody can be used for detection of LEF1 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only

Handling

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Images back to top
Format Lyophilized
Reconstitution Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store LEF1 antibody at -20 °C.

Images

Product Details anti-LEF1 Antibody Target Details LEF1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-LEF1 antibody (Lymphoid Enhancer-Binding Factor 1) (ABIN2460458) anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) antibody