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anti-Cow (Bovine) PKC beta antibody for ELISA

Recommended PKC beta Antibody (supplied by: Log in to see )

Antigen
Protein Kinase C, beta (PRKCB) Antibodies
  • A130082F03Rik
  • PKC
  • PKC-B
  • PKC-Beta
  • PKC-beta
  • PKCB
  • Pkcb
  • Prkcb
  • Prkcb1
  • PRKCB1
  • prkcb1
  • PRKCB2
  • Prkcb2
  • zgc:63591
Epitope
Internal Region
44
22
17
12
11
10
8
7
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
131
77
72
12
8
6
5
2
1
1
Host
Rabbit
148
13
7
7
Clonality
Polyclonal
Conjugate
This PKC beta antibody is un-conjugated
10
9
5
5
5
5
2
Application
ELISA, Western Blotting (WB)
151
95
61
22
15
8
6
5
3
3
3
2
1
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Available images

Catalog No. ABIN259923
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Similar anti-PKC beta Antibodies

Application / Reactivity Cow (Bovine)
ELISA 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Western Blotting (WB) 7 Antibodies

General

Antigen Protein Kinase C, beta (PRKCB) Antibodies
Epitope Internal Region
(44), (22), (17), (12), (11), (10), (8), (7), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(131), (77), (72), (12), (8), (6), (5), (2), (1), (1)
Host Rabbit
(148), (13), (7), (7)
Clonality
Polyclonal
Conjugate This PKC beta antibody is un-conjugated
(10), (9), (5), (5), (5), (5), (2)
Application ELISA, Western Blotting (WB)
(151), (95), (61), (22), (15), (8), (6), (5), (3), (3), (3), (2), (1)
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Product Details anti-PKC beta Antibody

Target Details PKC beta Application Details Handling Images
Specificity This antibody is specific for human PKC beta protein. A BLAST analysis was used to suggest cross-reactivity with PKC beta from human and monkey sources based on a 100% identity with the immunizing sequence. Expect partial cross reactivity to PKC beta from rabbit and bovine (93% identity) and rat and mouse (87% identity) sources. Cross-reactivity with PKC beta from other sources has not been determined.
Cross-Reactivity (Details) Predicted cross-reactivity based on sequence identity: Monkey (100%), Rabbit (93%), Bovine (93%), rat,(87%), Mouse (87%). Cross-reactivity with PKC beta from other sources has not been determined.
Purification Affinity purified
Immunogen This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Protein kinase C beta.

Target Details PKC beta

Product Details anti-PKC beta Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKC beta (PRKCB Antibody Abstract)
Background Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cellproliferation and angiogenesis. PKC is a family of serine- and threonine-specific proteinkinases that can be activated by calcium and second messenger diacylglycerol. PKCfamily members phosphorylate a wide variety of protein targets and are known to beinvolved in diverse cellular signaling pathways. PKC family members also serve asmajor receptors for phorbol esters, a class of tumor promoters. Each member of thePKC family has a specific expression profile and is believed to play a distinct role incells. The protein encoded by this gene is one of the PKC family members. This proteinkinase has been reported to be involved in many different cellular functions, such as Bcell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugarabsorption. Studies in mice also suggest that this kinase may also regulate neuronalfunctions and correlate fear-induced conflict behavior after stress. Alternatively splicedtranscript variants encoding distinct isoforms have been reported. Alternate Names: anti-MGC41878 antibody, anti-PKC B antibody, anti-PKC beta antibody, anti-PKCBantibody, anti-PRKC B antibody, anti-PRKC B1 antibody, anti-PRKCB antibody,anti-PRKCB1 antibody, anti-PRKCB2 antibody, anti-Protein kinase C beta 1 antibody,anti-Protein kinase C beta type antibody.
Gene Symbol: PRKCB
Gene ID 5579, 18751, 293508
Pathways WNT Signaling

Application Details

Product Details anti-PKC beta Antibody Target Details PKC beta Handling Images back to top
Application Notes This affinity purified antibody has been tested for use in ELISA and western blotting. Expect a band approximately 76 kDa in size corresponding - PKC beta by western blotting in the appropriate cell lysate or extract. Lysates from u251or BxPC3 cells are suggested for western blotting.
Recommended dilutions: ELISA 1:75000-1:80000, Western Blot 1:1000-1:2000
Restrictions For Research Use only

Handling

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Images back to top
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

Images

Product Details anti-PKC beta Antibody Target Details PKC beta Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PKC beta antibody (Protein Kinase C, beta) (Internal Region) (ABIN259923) Western blot using ABIN259923 shows detection of PKC beta in ~25 ug of U251 whole cel...