|Application / Reactivity||Mouse (Murine)||Human|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies||1 Antibodies|
|ELISA||35 Antibodies||48 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||33 Antibodies||33 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies||26 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||64 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Antigen||Protein Kinase C, gamma (PRKCG) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This PKC gamma antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PKC gamma AntibodyTarget Details PKC gamma Application Details Handling References for anti-PKC gamma Antibody (ABIN359066) Images
|Specificity||This antibody reacts to PKC gamma.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PKC.|
Target Details PKC gammaProduct Details anti-PKC gamma Antibody Application Details Handling References for anti-PKC gamma Antibody (ABIN359066) Images back to top
|Alternative Name||PRKCG (PRKCG Antibody Abstract)|
|Background||Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).Synonyms: PKC gamma, PKC-gamma, PKCC, PKCG, Protein kinase C gamma type|
|Gene ID||5582, 9606|
|Pathways||WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PKC gamma Antibody Target Details PKC gamma Handling References for anti-PKC gamma Antibody (ABIN359066) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PKC gamma Antibody Target Details PKC gamma Application Details References for anti-PKC gamma Antibody (ABIN359066) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PKC gamma Antibody (ABIN359066)Product Details anti-PKC gamma Antibody Target Details PKC gamma Application Details Handling Images back to top
van de Warrenburg, Verbeek, Piersma et al.: "Identification of a novel SCA14 mutation in a Dutch autosomal dominant cerebellar ataxia family." in: Neurology, Vol. 61, Issue 12, pp. 1760-5, 2003 (PubMed).
Yabe, Sasaki, Chen et al.: "Spinocerebellar ataxia type 14 caused by a mutation in protein kinase C gamma." in: Archives of neurology, Vol. 60, Issue 12, pp. 1749-51, 2003 (PubMed).
Uchino, Sakai, Kashiwagi et al.: "Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC." in: The Journal of biological chemistry, Vol. 279, Issue 3, pp. 2254-61, 2004 (PubMed).
Ananthanarayanan, Stahelin, Digman et al.: "Activation mechanisms of conventional protein kinase C isoforms are determined by the ligand affinity and conformational flexibility of their C1 domains." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46886-94, 2003 (PubMed).
Correia, Duarte, Faro et al.: "Protein kinase C gamma associates directly with the GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor subunit. Effect on receptor phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 8, pp. 6307-13, 2003 (PubMed).
ImagesProduct Details anti-PKC gamma Antibody Target Details PKC gamma Application Details Handling References for anti-PKC gamma Antibody (ABIN359066) back to top