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anti-Chemical Rhodopsin antibody for Western Blotting

Recommended Rhodopsin Antibody (supplied by: Log in to see )

Antigen
Rhodopsin (RHO) Antibodies
  • 1F9
  • 143283_at
  • BEST:GH11778
  • CG4550
  • CG5192
  • CG5279
  • CG9668
  • CG10888
  • CSNBAD1
  • DMELRH1
  • DMELRH3
  • DMELRH4
  • DMELRH5
  • DMELRH6
  • Dmel\\CG4550
  • Dmel\\CG5192
  • Dmel\\CG5279
  • Dmel\\CG9668
  • Dmel\\CG10888
  • Dm Rh1
  • DmRh1
  • Dm Rh3
  • Dm Rh4
  • Dm Rh5
  • Dm Rh6
  • DRh1
  • FBgn0002940
  • FBgn0003249
  • FBgn0003250
  • fi06d11
  • kfh-rh
  • NinaE
  • Nina E
  • Noerg1
  • opn2
  • Opn2
  • OPN2
  • ops
  • Ops
  • opsin
  • ora
  • PIGRHO1
  • R
  • R8
  • RDP1
  • Rh
  • Rh-1
  • RH1
  • rh1
  • Rh1
  • Rh1(ninaE)
  • Rh1/ninaE
  • rh1/ninaE
  • RH3
  • rh3
  • RH4
  • rh4
  • RH5
  • rh5
  • RH6
  • rh6
  • RHO
  • rhodopsin
  • RP4
  • rp4
  • wu:fi06d11
  • xrho
  • zfo2
  • zfrho
Reactivity
Chemical
147
38
23
13
5
4
3
2
1
1
1
Host
Mouse
176
39
3
1
Clonality
Monoclonal
Conjugate
This Rhodopsin antibody is un-conjugated
11
10
9
8
7
6
6
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
208
137
133
131
116
102
42
4
4
1
1
Supplier
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Catalog No. ABIN863085
$ 383.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN267076 ICC IF IHC (p) IHC WB Mouse IgG1 Log in to see RET-P1 1
0.124960825 ABIN487512 EIA IHC (fro) IF IP WB Mouse IgG1 C-Term Log in to see 1D4
0.124960825 ABIN487513 EIA IHC (fro) IF IP WB Mouse IgG1 C-Term Log in to see 1D4
0.124960825 ABIN3186758 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.124960825 ABIN3186759 ELISA IHC WB Rabbit IgG Thr789 Log in to see Polyclonal
0.124960825 ABIN4350402 ELISA ICC IF IHC IHC (p) IP WB Mouse IgG1 C-Term Log in to see 1D4
0.124960825 ABIN4350422 ICC IF WB Mouse IgG1 kappa Log in to see A531
0.124960825 ABIN4350424 ICC IF WB Mouse IgG1 kappa Log in to see B630
0.124960825 ABIN3032473 ELISA WB Rabbit Ig Fraction AA 310-339 Log in to see Polyclonal
0.0012207722 ABIN180439 IF IHC (p) WB Mouse IgG1 Log in to see RET-P1 4
0.0012207722 ABIN272076 IHC (p) WB Rabbit Log in to see Polyclonal
0.0012207722 ABIN863083 ICC IF IP IHC ELISA WB Mouse Log in to see 4D2 4
0.0012207722 ABIN487511 EIA IHC (fro) IF IP WB Mouse IgG1 N-Term Log in to see 4D2
0.0012207722 ABIN487510 EIA IHC (fro) IF IP WB Mouse IgG1 N-Term Log in to see 4D2
0.0012207722 ABIN2496531 IHC WB Mouse IgG1 Log in to see 1D4
0.0012207722 ABIN350678 IHC WB Rabbit 4th Cytoplasmic Loop Log in to see Polyclonal 10
0.0012207722 ABIN350798 IHC WB Rabbit IgG C-Term Log in to see Polyclonal 10
0.0012207722 ABIN350797 IHC WB Rabbit C-Term Log in to see Polyclonal 10
0.0012207722 ABIN2482252 ICC IF IP IHC ELISA WB Atto 390 Mouse Log in to see 4D2
0.0012207722 ABIN2482255 ICC IF IP IHC ELISA WB Atto 594 Mouse Log in to see 4D2

Top referenced anti-Rhodopsin antibody for Western Blotting

Similar anti-Rhodopsin Antibodies

Application / Reactivity Chemical
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 102 Antibodies
Immunofluorescence (IF) 110 Antibodies
Immunohistochemistry (IHC) 89 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 69 Antibodies
Western Blotting (WB) 141 Antibodies

General

Antigen Rhodopsin (RHO) Antibodies
Reactivity Chemical
(147), (38), (23), (13), (5), (4), (3), (2), (1), (1), (1)
Host Mouse
(176), (39), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Rhodopsin antibody is un-conjugated
(11), (10), (9), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(208), (137), (133), (131), (116), (102), (42), (4), (4), (1), (1)
Pubmed 4 references available
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Product Details anti-Rhodopsin Antibody

Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin Antibody (ABIN863085) Images
Specificity Detects ~40 kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Sensitivity 1 µg/mL of SMC-177 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Immunogen Bovine Rhodopsin
Clone 1D4

Target Details Rhodopsin

Product Details anti-Rhodopsin Antibody Application Details Handling References for anti-Rhodopsin Antibody (ABIN863085) Images back to top
Antigen
Alternative Name Rhodopsin (RHO Antibody Abstract)
Background Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Cellular Localization: Membrane
Gene ID 509933
NCBI Accession NP_001014890
UniProt P02699
Research Area Neurology
Pathways WNT Signaling

Application Details

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling References for anti-Rhodopsin Antibody (ABIN863085) Images back to top
Application Notes Recommended Dilution: WB (1:1000), IHC (1:100), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details References for anti-Rhodopsin Antibody (ABIN863085) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-Rhodopsin Antibody (ABIN863085)

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling Images back to top
Product cited in:

Cheng, Djajadi, Molday: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." in: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, 2012

Background publications

Iannaccone, Man, Waseem, Jennings, Ganapathiraju, Gallaher, Reese, Bhattacharya, Klein-Seetharaman: "Retinitis pigmentosa associated with rhodopsin mutations: Correlation between phenotypic variability and molecular effects." in: Vision research, Vol. 46, Issue 27, pp. 4556-67, 2006

Ridge, Lee, Abdulaev: "Examining rhodopsin folding and assembly through expression of polypeptide fragments." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7860-7, 1996

Molday, Hicks, Molday: "Peripherin. A rim-specific membrane protein of rod outer segment discs." in: Investigative ophthalmology & visual science, Vol. 28, Issue 1, pp. 50-61, 1987

Images

Product Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling References for anti-Rhodopsin Antibody (ABIN863085) back to top
Supplier Images
Western Blotting (WB) image for anti-Rhodopsin antibody (RHO) (ABIN863085) Rho Human Cell Line Mix, Western Blotting.