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anti-Human WNT3 antibody for Immunocytochemistry

Recommended WNT3 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
  • Ci-Wnt3
  • Int-4
  • INT4
  • int4
  • wnt-3
  • Wnt-3
  • wnt3
  • WNT3
  • wnt3-A
  • wnt3l
  • Xwnt-3
  • Xwnt3
Epitope
Internal Region
29
12
10
10
8
3
2
1
1
1
Reactivity
Human
74
13
9
6
1
1
1
1
Host
Goat
35
20
19
Clonality
Polyclonal
Conjugate
This WNT3 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
67
35
19
11
11
8
3
2
1
Supplier
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Available images

Catalog No. ABIN251471
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010194078 ABIN2466453 ICC IF IHC ELISA WB Goat Internal Region Log in to see Polyclonal
0.0010194078 ABIN2560784 ICC IF IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.0010194078 ABIN1872358 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Similar anti-WNT3 Antibodies

Application / Reactivity Human
ELISA 36 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
Epitope Internal Region
(29), (12), (10), (10), (8), (3), (2), (1), (1), (1)
Reactivity Human
(74), (13), (9), (6), (1), (1), (1), (1)
Host Goat
(35), (20), (19)
Clonality
Polyclonal
Conjugate This WNT3 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(67), (35), (19), (11), (11), (8), (3), (2), (1)
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Product Details anti-WNT3 Antibody

Target Details WNT3 Application Details Handling Images
Cross-Reactivity (Details) Expected cross reactivity from sequence similarity: Rat,Dog.
Purification affinity purified
Immunogen Synthetic peptide, CGRGHNTRTEKRKEK, representing the internal region of the human protein according to NP_110380.
Isotype IgG

Target Details WNT3

Product Details anti-WNT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Wnt3 (WNT3 Antibody Abstract)
Background The WNT gene family consists of structurally related genes which encode secretedsignaling proteins. These proteins have been implicated in oncogenesis and in severaldevelopmental processes, including regulation of cell fate and patterning duringembryogenesis. This gene is a member of the WNT gene family. It encodes a proteinwhich shows 98% amino acid identity to mouse Wnt3 protein, and 84% to humanWNT3A protein, another WNT gene product. The mouse studies show the requirementof Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggestthat this gene may play a key role in some cases of human breast, rectal, lung, andgastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. Thisgene is clustered with WNT15, another family member, in the chromosome 17q21region. Alternate Names: anti-WNT3 antibody, anti-wingless-type MMTV integration site family antibody,anti-member 3 antibody, anti-INT4 antibody, anti-MGC131950 antibody,anti-MGC138321 antibody, anti-MGC138323 antibody, anti-WNT-3 proto-oncogeneprotein precursor antibody.
Gene Symbol: WNT3
Gene ID 7473
NCBI Accession NP_110380
Pathways WNT Signaling

Application Details

Product Details anti-WNT3 Antibody Target Details WNT3 Handling Images back to top
Application Notes Approx 38kDa band observed in Human Brain lysates. This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J. 2011 Jul 11, PMID: 21746862.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 3-5µg/mL, Peptide ELISA detection limit 1:16000, Western Blot 1-3µg/mL
Restrictions For Research Use only

Handling

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris-saline [ pH 7.3] with 0.5 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (Internal Region) (ABIN251471) ABIN251471 (1?g/ml) staining of Human Brain lysate (35?g protein in RIPA buffer). Pr...