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anti-Human WNT3 antibody for Immunocytochemistry

Recommended WNT3 Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
  • Ci-Wnt3
  • WNT3
  • int4
  • Xwnt3
  • wnt-3
  • wnt3l
  • Xwnt-3
  • wnt3
  • Int-4
  • Wnt-3
  • INT4
  • wnt3-A
  • wingless-type MMTV integration site family, member 3
  • wingless-related MMTV integration site 3
  • WNT3
  • wnt3
  • Wnt3
Reactivity
Human, Mouse (Murine)
78
16
11
8
3
3
2
2
2
2
2
2
2
2
Host
Goat
37
21
20
Clonality
Polyclonal
Conjugate
This WNT3 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
72
36
20
12
10
8
4
2
1
1
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Catalog No. ABIN251471
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2466453 ICC IF IHC ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2560784 ICC IF IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1872358 ICC IHC IP WB Rabbit IgG AA 59-355 Log in to see Polyclonal
1 ABIN5080548 ICC IF Rabbit IgG Log in to see Polyclonal

Top referenced anti-WNT3 antibody for Immunocytochemistry

Similar anti-WNT3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 9 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 5 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 11 Antibodies
Western Blotting (WB) 14 Antibodies 73 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 3 (WNT3) Antibodies
Reactivity Human, Mouse (Murine)
(78), (16), (11), (8), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Host Goat
(37), (21), (20)
Clonality
Polyclonal
Conjugate This WNT3 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(72), (36), (20), (12), (10), (8), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-WNT3 Antibody

Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN251471) Images
Purification Immunogen affinity purified
Immunogen Peptide with sequence CGRGHNTRTEKRKEK corresponding to internal region according to NP_110380.1.

Target Details WNT3

Product Details anti-WNT3 Antibody Application Details Handling References for anti-WNT3 Antibody (ABIN251471) Images back to top
Antigen
Alternative Name Wnt3 (WNT3 Antibody Abstract)
Background Gene Symbol: WNT3
Gene ID 7473
NCBI Accession NP_110380
Research Area Stem Cells, Wnt, Neurogenesis
Pathways WNT Signaling, Regulation of Cell Size

Application Details

Product Details anti-WNT3 Antibody Target Details WNT3 Handling References for anti-WNT3 Antibody (ABIN251471) Images back to top
Application Notes Western Blot 1 - 3 μg/mL, Immunohistochemistry 2 - 4 μg/mL, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 3 - 5 μg/mL, Peptide ELISA Detection limit 1:16000Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1). IHC: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. ICC/IF successful usage for mouse hippocampal primary astrocytes is reported in scientific literature (PMID: 21746862).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details References for anti-WNT3 Antibody (ABIN251471) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.5 mg/mL Tris ( pH 7.3) and 0.5 % BSA
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References for anti-WNT3 Antibody (ABIN251471)

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling Images back to top
Product cited in:

Okamoto, Inoue, Iwamura, Terashima, Soya, Asashima, Kuwabara: "Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 10, pp. 3570-82, 2011

Schmitt, Shi, Wolf, Lu, King, Zou: "Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping." in: Nature, Vol. 439, Issue 7072, pp. 31-7, 2006

Images

Product Details anti-WNT3 Antibody Target Details WNT3 Application Details Handling References for anti-WNT3 Antibody (ABIN251471) back to top
Supplier Images
Western Blotting (WB) image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (ABIN251471) ABIN251471 (1?g/ml) staining of Human Brain lysate (35?g protein in RIPA buffer). Pr...
Western Blotting (WB) image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (ABIN251471) Western Blot: Wnt3 Antibody [ABIN2514713] - Analysis of Wnt3 at 1ug/ml in Human Brain...
Western Blotting (WB) image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (ABIN251471) Western Blot: Wnt3 Antibody [ABIN2514713] - (1ug/ml) staining of lysate of cell line ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (ABIN251471) Immunohistochemistry-Paraffin: Wnt3 Antibody [ABIN2514713] - (3.8ug/ml) staining of p...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-WNT3 antibody (Wingless-Type MMTV Integration Site Family, Member 3) (ABIN251471) Immunohistochemistry-Paraffin: Wnt3 Antibody [ABIN2514713] - Human Breast.