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anti-Human WNT5A antibody for Immunocytochemistry

Recommended WNT5A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
  • 8030457G12Rik
  • hWNT5A
  • wnt-5A
  • Wnt-5a
  • WNT5A
  • wnt5a-B
Reactivity
Human
86
35
24
13
1
1
1
1
1
Host
Mouse
66
30
1
1
Clonality
Monoclonal
Conjugate
This WNT5A antibody is un-conjugated
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
75
51
48
19
16
11
10
2
1
1
1
1
1
Supplier
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Catalog No. ABIN969460
$ 393.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.6409031 ABIN1846736 ICC IF IHC ELISA WB Mouse IgG1 Log in to see

Top referenced anti-WNT5A antibody for Immunocytochemistry

Similar anti-WNT5A Antibodies

Application / Reactivity Human
ELISA 41 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 48 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 18 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 70 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
Reactivity Human
(86), (35), (24), (13), (1), (1), (1), (1), (1)
Host Mouse
(66), (30), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This WNT5A antibody is un-conjugated
(5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(75), (51), (48), (19), (16), (11), (10), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-WNT5A Antibody

Target Details WNT5A Application Details Handling References for anti-WNT5A Antibody (ABIN969460) Images
Purification Ascites
Immunogen Purified recombinant fragment of WNT5A expressed in E. coli.
Clone 6F2
Isotype IgG1

Target Details WNT5A

Product Details anti-WNT5A Antibody Application Details Handling References for anti-WNT5A Antibody (ABIN969460) Images back to top
Antigen
Alternative Name WNT5A (WNT5A Antibody Abstract)
Background WNT5A: wingless-type MMTV integration site family, member 5A. Entrez Protein: NP_003383. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98 % , 98 % and 87 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
Molecular Weight 42.3 kDa
Gene ID 7474
HGNC 7474
Pathways WNT Signaling

Application Details

Product Details anti-WNT5A Antibody Target Details WNT5A Handling References for anti-WNT5A Antibody (ABIN969460) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details References for anti-WNT5A Antibody (ABIN969460) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-WNT5A Antibody (ABIN969460)

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling Images back to top
Product cited in:

Ford, Ekström, Andersson: "Wnt-5a signaling restores tamoxifen sensitivity in estrogen receptor-negative breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 10, pp. 3919-24, 2009

Dai, Hall, Escara-Wilke, Mizokami, Keller, Keller: "Prostate cancer induces bone metastasis through Wnt-induced bone morphogenetic protein-dependent and independent mechanisms." in: Cancer research, Vol. 68, Issue 14, pp. 5785-94, 2008

Images

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling References for anti-WNT5A Antibody (ABIN969460) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (ABIN969460) Figure 1: Confocal immunofluorescence analysis of PC-12 cells using WNT5A mouse mAb (...
Immunohistochemistry (IHC) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (ABIN969460) Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), th...
Western Blotting (WB) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (ABIN969460) Figure 3: Western blot analysis using WNT5A mouse mAb against HEK293 (1) and WNT5A-hI...