anti-Human WNT5A antibody for Immunohistochemistry

Recommended WNT5A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
  • hWNT5A
  • wnt-5A
  • wnt5a-B
  • WNT5A
  • 8030457G12Rik
  • Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • wingless-type MMTV integration site family, member 5a
  • wingless-related MMTV integration site 5A
  • Wnt signaling ligand
  • WNT-5A protein
  • WNT5A
  • Wnt5a
  • wnt5a
  • LOC578814
  • wnt5
  • WNT-5A
Epitope
Middle Region
15
14
11
7
5
5
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
118
45
33
17
4
2
2
2
2
1
1
1
Host
Rabbit
71
55
2
1
Clonality
Polyclonal
Conjugate
This WNT5A antibody is un-conjugated
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
92
60
54
47
17
13
10
4
2
1
1
1
1
1
Options
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Catalog No. ABIN2779470
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.649799 ABIN969461 IHC ELISA WB Mouse IgG1 Log in to see 3D10 2
9.649799 ABIN969460 ICC IHC ELISA WB Mouse IgG1 Log in to see 6F2 2
9.649799 ABIN2470815 IHC ELISA WB Mouse IgG1 Log in to see 3D10
9.649799 ABIN1875353 IHC WB Rabbit IgG Log in to see Polyclonal
9.649799 ABIN2470810 IF IHC ELISA WB Mouse IgG1 Log in to see 6F2
9.649799 ABIN931340 IHC ELISA WB Mouse IgG1 Log in to see 3D10
9.649799 ABIN3029634 FACS IHC ELISA WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal
9.649799 ABIN1846736 ELISA IF/ICC IHC WB Mouse IgG1 Log in to see
9.649799 ABIN1846737 IHC ELISA WB Mouse IgG1 Log in to see
1 ABIN4366183 ELISA IHC IHC (p) WB Mouse IgG1 Log in to see 3D10 1
1 ABIN2869402 ELISA ICC IHC WB Mouse IgG1 Log in to see 6F2 3
1 ABIN2869410 ELISA IHC WB Mouse IgG1 Log in to see 3D10 2
1 ABIN449945 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 6F2
1 ABIN4366186 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN967259 IF IHC ELISA WB Mouse IgG1 Log in to see 6F2 3
1 ABIN3023003 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4366188 IHC IHC (p) WB Rabbit Log in to see Polyclonal 9
1 ABIN2570931 ELISA FACS IHC WB Rabbit IgG AA 192-221 Log in to see Polyclonal
1 ABIN1948763 FACS IHC ELISA WB APC Rabbit IgG AA 192-221 Log in to see Polyclonal
1 ABIN1948762 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 192-221 Log in to see Polyclonal

Top referenced anti-WNT5A antibody for Immunohistochemistry

Similar anti-WNT5A Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Rabbit Rat (Rattus) Dog (Canine) Guinea Pig Horse (Equine) Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies 1 Antibodies
Western Blotting (WB) 82 Antibodies 5 Antibodies 34 Antibodies 3 Antibodies 22 Antibodies 6 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 4 Antibodies 10 Antibodies 11 Antibodies 5 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
Epitope Middle Region
(15), (14), (11), (7), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(118), (45), (33), (17), (4), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(71), (55), (2), (1)
Clonality
Polyclonal
Conjugate This WNT5A antibody is un-conjugated
(6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(92), (60), (54), (47), (17), (13), (10), (4), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-WNT5A Antibody

Target Details WNT5A Application Details Handling ProductDetails: References for anti-WNT5A antibody (ABIN2779470) Images
Blocking Peptide Blocking peptide for this product available: ABIN986222
Sequence GGCGDNIDYG YRFAKEFVDA RERERIHAKG SYESARILMN LHNNEAGRRT
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 87%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 80%
Characteristics This is a rabbit polyclonal antibody against WNT5A. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human WNT5A

Target Details WNT5A

Product Details anti-WNT5A Antibody Application Details Handling ProductDetails: References for anti-WNT5A antibody (ABIN2779470) Images back to top
Antigen
Alternative Name WNT5A (WNT5A Antibody Abstract)
Background The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT5A is a member of the WNT protein family. It shows 98 % , 98 % and 87 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98 %, 98 % and 87 % amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: hWNT5A
Protein Interaction Partner: UBC, LRP6, FZD2, FZD1, ROR2, FZD5,
Protein Size: 380
Molecular Weight 42 kDa
Gene ID 7474
NCBI Accession NM_003392, NP_003383
UniProt P41221
Pathways WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation

Application Details

Product Details anti-WNT5A Antibody Target Details WNT5A Handling ProductDetails: References for anti-WNT5A antibody (ABIN2779470) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 380 AA

Restrictions For Research Use only

Handling

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details ProductDetails: References for anti-WNT5A antibody (ABIN2779470) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-WNT5A antibody (ABIN2779470)

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling Images back to top
Background publications

Witze, Litman, Argast, Moon, Ahn: "Wnt5a control of cell polarity and directional movement by polarized redistribution of adhesion receptors." in: Science (New York, N.Y.), Vol. 320, Issue 5874, pp. 365-9, 2008

Images

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling ProductDetails: References for anti-WNT5A antibody (ABIN2779470) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (Middle Region) (ABIN2779470) anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (Middle Region) antibody
Immunohistochemistry (IHC) image for anti-WNT5A antibody (Wingless-Type MMTV Integration Site Family, Member 5A) (Middle Region) (ABIN2779470) anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (Middle Region) antibody (Image 2)