anti-Human WNT5A antibody for Western Blotting

Recommended WNT5A Antibody (supplied by: Log in to see )

Antigen
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
  • hWNT5A
  • wnt-5A
  • wnt5a-B
  • WNT5A
  • 8030457G12Rik
  • Wnt-5a
  • wingless-type MMTV integration site family, member 5A
  • wingless-type MMTV integration site family, member 5a
  • wingless-related MMTV integration site 5A
  • Wnt signaling ligand
  • WNT-5A protein
  • WNT5A
  • Wnt5a
  • wnt5a
  • LOC578814
  • wnt5
  • WNT-5A
Epitope
AA 185-213, Center
15
14
10
7
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
119
46
34
18
5
3
3
2
2
1
1
1
1
1
Host
Rabbit
71
56
2
1
Clonality
Polyclonal
Conjugate
This WNT5A antibody is un-conjugated
6
5
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
93
62
55
46
16
13
11
4
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN651869
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9066148 ABIN3043962 IHC (p) WB Rabbit IgG AA 184-303 Log in to see Polyclonal
2.9066148 ABIN675758 IF (p) IHC (p) WB Rabbit IgG AA 330-380 Log in to see Polyclonal 2
2.9066148 ABIN4366183 ELISA IHC IHC (p) WB Mouse IgG1 Log in to see 3D10 1
2.9066148 ABIN969461 IHC ELISA WB Mouse IgG1 Log in to see 3D10 2
2.9066148 ABIN969460 ICC IHC ELISA WB Mouse IgG1 Log in to see 6F2 2
2.9066148 ABIN2854813 WB Rabbit IgG Center Log in to see Polyclonal
2.9066148 ABIN1109514 IHC (p) WB Mouse IgG1 Log in to see 3D10 1
2.9066148 ABIN1109513 EIA IF IHC (p) WB Mouse IgG1 Log in to see 6F2 1
2.9066148 ABIN449945 ICC IF IHC (p) IHC ELISA WB Mouse IgG1 Log in to see 6F2
2.9066148 ABIN4366186 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
2.9066148 ABIN3023003 IHC WB Rabbit IgG Log in to see Polyclonal
2.9066148 ABIN1875353 IHC WB Rabbit IgG Log in to see Polyclonal
2.9066148 ABIN635529 WB Rabbit Middle Region Log in to see Polyclonal
2.9066148 ABIN3029634 FACS IHC ELISA WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal
2.9066148 ABIN931340 IHC ELISA WB Mouse IgG1 Log in to see 3D10
2.9066148 ABIN5534831 FACS IHC (p) WB Rabbit Ig Fraction AA 185-213 Log in to see Polyclonal
2.9066148 ABIN521384 PLA WB Rabbit AA 1-380, full length Log in to see Polyclonal
2.9066148 ABIN3029635 IHC (p) WB Rabbit IgG AA 184-303 Log in to see Polyclonal
2.9066148 ABIN5545279 WB Rabbit IgG Log in to see Polyclonal
2.9066148 ABIN1846736 ELISA IF/ICC IHC WB Mouse IgG1 Log in to see

General

Antigen Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) Antibodies
Epitope AA 185-213, Center
(15), (14), (10), (7), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(119), (46), (34), (18), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(71), (56), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This WNT5A antibody is un-conjugated
(6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(93), (62), (55), (46), (16), (13), (11), (4), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-WNT5A Antibody

Target Details WNT5A Application Details Handling References for anti-WNT5A antibody (ABIN651869) Images
Specificity This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-221 amino acids from the Central region of human WNT5A.
Predicted Reactivity Mouse (Murine),Rat (Rattus),Rabbit
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This WNT5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-213 AA from the Central region of human WNT5A.
Clone RB22340
Isotype Ig

Target Details WNT5A

Product Details anti-WNT5A Antibody Application Details Handling References for anti-WNT5A antibody (ABIN651869) Images back to top
Antigen
Alternative Name WNT5A (WNT5A Antibody Abstract)
Background The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq].
Synonyms: Protein Wnt-5a,WNT5A,
Molecular Weight 42339 DA
Gene ID 7474
NCBI Accession NP_003383
UniProt P41221
Research Area Cell Signaling, Protein Modifications, Cell Cycle, Cell Structure
Pathways WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation

Application Details

Product Details anti-WNT5A Antibody Target Details WNT5A Handling References for anti-WNT5A antibody (ABIN651869) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details References for anti-WNT5A antibody (ABIN651869) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-WNT5A antibody (ABIN651869)

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling Images back to top
Background publications

He, Saint-Jeannet, Wang, Nathans, Dawid, Varmus: "A member of the Frizzled protein family mediating axis induction by Wnt-5A." in: Science (New York, N.Y.), Vol. 275, Issue 5306, pp. 1652-4, 1997

Danielson, Pillarisetti, Cohen, Sholehvar, Huebner, Ng, Nicholls, Cheah, Iozzo: "Characterization of the complete genomic structure of the human WNT-5A gene, functional analysis of its promoter, chromosomal mapping, and expression in early human embryogenesis." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31225-34, 1996

Smolich, McMahon, McMahon, Papkoff: "Wnt family proteins are secreted and associated with the cell surface." in: Molecular biology of the cell, Vol. 4, Issue 12, pp. 1267-75, 1994

Clark, Cohen, Eichstetter, Cannizzaro, McPherson, Wasmuth, Iozzo: "Molecular cloning of the human proto-oncogene Wnt-5A and mapping of the gene (WNT5A) to chromosome 3p14-p21." in: Genomics, Vol. 18, Issue 2, pp. 249-60, 1994

Images

Product Details anti-WNT5A Antibody Target Details WNT5A Application Details Handling References for anti-WNT5A antibody (ABIN651869) back to top
Supplier Images
Western Blotting (WB) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869) WNT5A Antibody (Center) (ABIN651869) western blot analysis in Hela cell line lysates ...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869) WNT5A Antibody (Center) (ABIN651869) immunohistochemistry analysis in formalin fixed ...
Flow Cytometry (FACS) image for anti-Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 185-213), (Center) antibody (ABIN651869) WNT5A Antibody (Center) (ABIN651869) flow cytometric analysis of Hela cells (right hi...