anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) Antibodies

GAD2 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. Additionally we are shipping GAD65 Kits (41) and GAD65 Proteins (21) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
GAD2 24380 Q05683
GAD2 2572 Q05329
GAD2 14417 P48320
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Top anti-GAD65 Antibodies at antibodies-online.com

Showing 10 out of 230 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IHC (p), WB Anti-GAD65 antibody, IHC(P) IHC(P): Rat Brain Tissue Anti-GAD65 antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Brain Tissue Lysate 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit Un-conjugated IHC (p), WB GAD65 was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- GAD65 Antigen Affinity purified polyclonal antibody (Catalog #) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). GAD65 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- GAD65 Antigen Affinity purified polyclonal antibody (Catalog #) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Goat Un-conjugated ELISA, WB ABIN185388 (0.1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 100 μg Log in to see 6 to 7 Days
$429.84
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Sample (50 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000. Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500. 100 μL Log in to see 8 to 11 Days
$559.35
Details
Human Mouse Un-conjugated ELISA, FACS, WB Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng); Dilution: 1/10000 Figure 2:Western blot analysis using GAD2 mAb against human GAD2 (AA: 1-100) recombinant protein. (Expected MW is 36.3 kDa) Dilution: 1/500 - 1/2000 100 μL Log in to see 8 to 11 Days
$577.50
Details
Mouse Mouse Un-conjugated FACS, IP, RIA, WB 0.1 mL Log in to see 7 to 8 Days
$510.24
Details
Human Goat Un-conjugated ELISA, IHC, IHC (p), WB Human Brain, cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 11 to 14 Days
$484.00
Details
Human Rabbit Un-conjugated IF, IHC (p), WB GAD2 Antibody (Center)  western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow). GAD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry.  Clinical relevance has not been evaluated. 400 μL Log in to see 10 to 11 Days
$385.00
Details
Human Rabbit Un-conjugated IHC (p), WB WB Image Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE antibody diluted at 1:10000 IHC-P Image GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody [C2C3], C-term , dilution: 1:500. 100 μL Log in to see 3 to 4 Days
$466.18
Details
Human Goat Un-conjugated ELISA, WB Western Blot: GAD65 Antibody [ABIN250366] - ABIN250366 (0.1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Immunohistochemistry: GAD2/GAD65 Antibody  - (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. . 0.1 mg Log in to see 7 to 9 Days
$488.73
Details

Top referenced anti-GAD65 Antibodies

  1. Rat (Rattus) Monoclonal GAD65 Primary Antibody for ICC, IP - ABIN1742417 : Coley, Ruffin, Moss, Hopfer, Boron: Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons. in Neuroscience 2013 (PubMed)
    Show all 3 Pubmed References

  2. Mouse (Murine) Monoclonal GAD65 Primary Antibody for FACS, IP - ABIN1042632 : Steed, Gilliam, Harris, Lernmark, Hampe: Antigen presentation of detergent-free glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier protein. in Journal of immunological methods 2008 (PubMed)
    Show all 3 Pubmed References

  3. Rat (Rattus) Polyclonal GAD65 Primary Antibody for ICC, ELISA - ABIN1742419 : Saliba, Gu, Yan, Moss: Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition. in The Journal of biological chemistry 2009 (PubMed)
    Show all 2 Pubmed References

  4. Rat (Rattus) Polyclonal GAD65 Primary Antibody for ICC, IHC - ABIN1742418 : Kaczor, Rakus, Mozrzymas: Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes. in Frontiers in cellular neuroscience 2015 (PubMed)
    Show all 2 Pubmed References

  5. Human Polyclonal GAD65 Primary Antibody for IHC (p), WB - ABIN3044396 : Shi, Ma, Zheng, Ruan, Liu, Tian, Zhang, He, Li: Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum. in Toxicology and industrial health 2012 (PubMed)
    Show all 2 Pubmed References

  6. Human Polyclonal GAD65 Primary Antibody for ICC, IF - ABIN4313137 : Grigoryan, Segal: Prenatal stress affects network properties of rat hippocampal neurons. in Biological psychiatry 2013 (PubMed)
    Show all 2 Pubmed References

  7. Human Monoclonal GAD65 Primary Antibody for IHC (p), IP - ABIN2473839 : Hampe, Lundgren, Daniels, Hammerle, Marcovina, Lernmark: A novel monoclonal antibody specific for the N-terminal end of GAD65. in Journal of neuroimmunology 2001 (PubMed)

  8. Dog (Canine) Polyclonal GAD65 Primary Antibody for ELISA, WB - ABIN547433 : Meyre, Boutin, Tounian, Deweirder, Aout, Jouret, Heude, Weill, Tauber, Tounian, Froguel: Is glutamate decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? in The Journal of clinical endocrinology and metabolism 2005 (PubMed)

  9. Hamster Polyclonal GAD65 Primary Antibody for IHC, IHC (p) - ABIN4313136 : Lee, Suk, Kang, McGeer, McGeer: Neurotoxic factors released by stimulated human monocytes and THP-1 cells. in Brain research 2011 (PubMed)

More Antibodies against GAD65 Interaction Partners

Rhesus Monkey Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. Results describe the gross distribution patterns of GAD65 in human and rhesus monkey thalami and show that they are nearly identical.

Zebrafish Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. GAD65 mRNA was found in the periaqueductal gray.

Human Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. In individuals with adult-onset diabetes, presence of N-terminally truncated GAD65 autoantibodies is associated with the clinical phenotype of autoimmune type 1 diabetes and predicts insulin therapy.

  2. This study showed a significant 58% increase of SCN GAD65/67-ir and a significant 169% increase of SCN GAD67-mRNA in the depression group.

  3. healthy donor NK cells showed similar degranulation against both GAD65 AA 114-122 pulsed and unpulsed APCs. The pathogenetic significance of the CD3-CD8dullCD56+ 'memory-like NK cell subset' with increased response upon secondary challenge in diabetics remains to be elucidated

  4. Genetic variability in GAD2 and GAD1 contributes to risk of methamphetamine dependence in the Thai population.

  5. Study propose that aberrant accumulation of immunogenic GAD65 in Golgi membranes facilitates inappropriate presentation to the immune system after release from stressed and/or damaged beta-cells, triggering autoimmunity.

  6. UV exposed hydrogen peroxide produces hydroxyl radical that may cause protein damage (GAD-65) to the extent of generating neo-epitopes on the molecule, making it immunogenic.

  7. we investigated the relationship between serum glutamic acid decarboxylase (GAD) autoantibody (Ab) levels and single nucleotide polymorphisms (SNPs) of the glutamic acid de-carboxylase 2 (GAD2) 5'-untranslated region

  8. GAD autoantibodies were associated with risk of developing type 1 diabetes.

  9. Kaplan-Meier survival curves estimated a worst pancreas graft survival for patients with positive IA-2 antibodies versus those patients with negative auto-antibodies and GAD65+ auto-antibodies after simultaneous pancreas kidney transplantation.

  10. Plasma GAD65 qualifies as a marker for early beta-cell loss after intraportal transplantation.

  11. Cohort study shows that mean GAD65 mRNA levels in the prefrontal cortex area 9 are not altered in subjects with schizophrenia but are lower in subjects with schizoaffective disorder

  12. Data suggest that women with gestational diabetes who develop autoantibodies against GAD2 (glutamate decarboxylase 2) exhibit higher blood glucose levels, insulin resistance, and impaired insulin secretion from beta cells in the postpartum period.

  13. Phosphate-activated glutaminase and GAD65/67 concentrations are compared in Alzheimer's disease cerebellum versus normal cerebellum controls

  14. GAD2 SNPs significantly associate with early-onset obsessive compulsive disorder.

  15. The decreased amount of GAD65 and GAD67 suggests the decreased synthesis of neurotransmitter and basic GABA pools that indicates insufficient functioning of the GABA system in the cerebellar cortex of Alzheimer's disease patients.

  16. There is a marked gender dimorphism in GAD65 positivity, higher in obstructive sleep apnoea syndrome females vs. males.

  17. GAD65 expression in 313 gastric cancer tissues was significantly higher than in 60 adjacent non-tumour tissues. Moreover, the expression level of GAD65 significantly correlated with the depth of tumour invasion and TNM stage.

  18. GAD65 is severely reduced in Alzheimer's disease middle temporal gyrus, hippocampus and putamen neuropil granules.

  19. The study demonstrated a statistically significant difference in the frequency of the GAD2 (+83987 T/A) genes between obese patients and controls in Taiwanese subjects.

  20. Decreased expression of GAD65 protein within inhibitory boutons could contribute to auditory impairments in schizophrenia.

Mouse (Murine) Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. These results thus identify a molecularly distinct, orexin-activated lateral hypothalamus submodule that governs physical activity in mice.

  2. Results suggest age-dependent decrease of GAD65/67 mRNAs but normal densities of certain GABAergic interneurons in the Shank3 transgenic mice.

  3. GABA is the major inhibitory neurotransmitter in the brain. We show that Dlx1/Dlx2 homeobox genes regulate GABA synthesis during forebrain development through direct activation of glutamic acid decarboxylase enzyme isoforms that convert glutamate to GABA.

  4. Treadmill running prevented partial sciatic nerve ligation-induced reductions in GAD65/67 production, and, thus, GABA levels may be retained in interneurons and neuropils in the superficial dorsal horn.

  5. Increased activity due to bicuculline preferentially up-regulated GAD65. This was reduced by AP5, a selective blocker of Ca2+-permeable ion channels and NMDA-Rs, and by U0126, KN93, and K252a, affecting BDNF pathways.

  6. Data show that miRNAs are involved in regulating the expression of the major type 1 diabetes (T1D) autoantigens IA-2, IA-2beta, and GAD65 enzyme.

  7. Results indicate the significant contribution of not only GAD65, GAD67 and VGAT-mediated GABAergic but also glycinergic transmissions to both palate and abdominal wall formations

  8. Analysis of GAD65/67 single and double knock-out embryos revealed that the two GADs play complementary (inhibitory) roles in GnRH migration ultimately modulating the speed and/or direction of GnRH migration.

  9. Results show that GAD65 is required for efficient GABAergic synaptic transmission and plasticity, and for maintaining extracellular GABA at a level needed for associative plasticity at cortical inputs in the lateral amygdala

  10. Menin regulates spinal glutamate-GABA balance through GAD65 contributing to neuropathic pain.

  11. Our data suggest a role of GAD65-mediated GABA synthesis in the encoding of circadian information to fear memory

  12. Autoimmunity to GAD65 may play a role in the development of Stiff person syndrome.

  13. Prenatal and postnatal maternal effects influence GAD65 and GAD67 gene expression depending on the age of the offspring.

  14. These findings indicate novel, differential roles for GluN2A, B and D receptors and for GAD65-mediated GABA in the regulation of individual topographies of orofacial movement

  15. SMAR1 and p53 act synergistically to up-regulate GAD65 expression upon Streptozotocin treatment.

  16. The mouse lateral striatum concentrates medium-sized projection neurons with high-level expression of GAD1, but not of GAD2, mRNA.

  17. Data show that chronic pain epigenetically suppresses Gad2 transcription through histone deacetylase-mediated histone hypoacetylation, resulting in impaired gamma-aminobutyric acid synaptic inhibition.

  18. These results suggest that modulation of the synaptic transmission from primary afferents to SG neurons by group III mGluR agonist is specific to the type of nociceptive primary afferents but not to the type of target neurons.

  19. Dissection of the intracellular pathway that underlies this process revealed that BDNF/TrkB signaling controls the transcription of GAD65

  20. GAD65 is crucial for maintenance of biosynthesis of synaptic GABA particularly by direct synthesis from astrocytic glutamine via glutamate.

GAD65 (GAD2) Antigen Profile

Protein Summary

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Gene names and symbols associated with GAD2

  • glutamate decarboxylase 2 (GAD2) antibody
  • glutamate decarboxylase 2 (PTRG_06148) antibody
  • glutamate decarboxylase 2 (LOC9324534) antibody
  • glutamate decarboxylase 2 (Gad2) antibody
  • glutamate decarboxylase 1 (GAD1) antibody
  • glutamate decarboxylase 2 (gad2) antibody
  • glutamic acid decarboxylase 2 (Gad2) antibody
  • 6330404F12Rik antibody
  • F12P19.12 antibody
  • F12P19_12 antibody
  • GAD(65) antibody
  • Gad-2 antibody
  • gad65 antibody
  • glutamate decarboxylase 2 antibody
  • zgc:112198 antibody

Protein level used designations for GAD2

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 , glutamic acid decarboxylase , 65 kDa glutamic acid decarboxylase , GAD-65 , Glutamate decarboxylase 2 (islet) , glutamate decarboxylase 65 kDa isoform , glutamic acid decarboxylase 2 , glutamic acid decarboxylase 65 , Glutamate decarboxylase-2 (pancreas)

GENE ID SPECIES
466026 Pan troglodytes
512459 Bos taurus
613029 Macaca mulatta
4836947 Scheffersomyces stipitis CBS 6054
6344405 Pyrenophora tritici-repentis Pt-1C-BFP
9324534 Arabidopsis lyrata subsp. lyrata
24380 Rattus norvegicus
100344727 Oryctolagus cuniculus
842908 Arabidopsis thaliana
550403 Danio rerio
2572 Homo sapiens
487107 Canis lupus familiaris
395395 Gallus gallus
396929 Sus scrofa
14417 Mus musculus
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