anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) Antibodies

GAD2 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes.

list all antibodies Gene Name GeneID UniProt
Anti-Rat GAD2 GAD2 24380 Q05683
Anti-Human GAD2 GAD2 2572 Q05329
Anti-Mouse GAD2 GAD2 14417 P48320
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Top anti-GAD65 Antibodies at antibodies-online.com

Showing 10 out of 255 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Sample (50 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000. Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500. 100 μL Log in to see 8 to 11 Days
$556.31
Details
Mouse Mouse Un-conjugated FACS, IP, RIA, WB 0.1 mL Log in to see 7 to 8 Days
$519.27
Details
Human Goat Un-conjugated ELISA, IHC, IHC (p), WB Human Brain, cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE) Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 7 to 9 Days
$484.00
Details
Human Rabbit Un-conjugated IHC (p), WB WB Image Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE antibody diluted at 1:10000 IHC-P Image GAD65 antibody [C2C3], C-term detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody [C2C3], C-term , dilution: 1:500. 100 μL Log in to see 1 to 2 Days
$358.60
Details
Human Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Mouse Cerebrum lysate; Primary Ab: 3µg/ml Rabbit Anti-Human GAD2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) 100 μg Log in to see 13 to 16 Days
$332.00
Details
Rat Rabbit Un-conjugated ICC, IHC, WB Figure. Western Blot; Sample: Rat Serum. 100 μg Log in to see 13 to 16 Days
$362.00
Details
Human Rabbit Un-conjugated IHC (p), WB GAD65 was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- GAD65 Antigen Affinity purified polyclonal antibody (Catalog #) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). GAD65 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- GAD65 Antigen Affinity purified polyclonal antibody (Catalog #) at 1 µg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022). 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit Un-conjugated IF, IHC (p), WB GAD2 Antibody (Center)  western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GAD2 antibody detected the GAD2 protein (arrow). GAD2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GAD2 Antibody (Center) for immunohistochemistry.  Clinical relevance has not been evaluated. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated ICC, IF, IHC (p), WB WB Image Sample (50 ug of whole cell lysate) A: rat brain 7.5% SDS PAGE antibody diluted at 1:1000 IHC-P Image GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody , dilution: 1:500. 100 μL Log in to see 1 to 2 Days
$358.60
Details
Human Rabbit Un-conjugated IP, WB GAD2 MaxPab rabbit polyclonal antibody. Western Blot analysis of GAD2 expression in mouse kidney. Immunoprecipitation of GAD2 transfected lysate using anti-GAD2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead , and immunoblotted with GAD2 purified MaxPab mouse polyclonal antibody (B01P) . 100 μL Log in to see 11 to 12 Days
$425.71
Details

Top referenced anti-GAD65 Antibodies

  1. Rat (Rattus) Monoclonal GAD65 Primary Antibody for ICC, IP - ABIN1742417 : Coley, Ruffin, Moss, Hopfer, Boron: Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons. in Neuroscience 2013 (PubMed)
    Show all 3 Pubmed References

  2. Mouse (Murine) Monoclonal GAD65 Primary Antibody for FACS, IP - ABIN1042632 : Steed, Gilliam, Harris, Lernmark, Hampe: Antigen presentation of detergent-free glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier protein. in Journal of immunological methods 2008 (PubMed)
    Show all 3 Pubmed References

  3. Rat (Rattus) Polyclonal GAD65 Primary Antibody for ICC, IHC - ABIN1742418 : Saliba, Gu, Yan, Moss: Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition. in The Journal of biological chemistry 2009 (PubMed)
    Show all 2 Pubmed References

  4. Rat (Rattus) Polyclonal GAD65 Primary Antibody for ICC, ELISA - ABIN1742419 : Du, Chazalon, Bestaven, Leste-Lasserre, Baufreton, Cazalets, Cho, Garret: Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease. in Neuroscience 2016 (PubMed)
    Show all 2 Pubmed References

  5. Human Polyclonal GAD65 Primary Antibody for ICC, IF - ABIN4313137 : Grigoryan, Segal: Prenatal stress affects network properties of rat hippocampal neurons. in Biological psychiatry 2013 (PubMed)
    Show all 2 Pubmed References

  6. Human Polyclonal GAD65 Primary Antibody for ELISA, WB - ABIN250366 : Meyre, Boutin, Tounian, Deweirder, Aout, Jouret, Heude, Weill, Tauber, Tounian, Froguel: Is glutamate decarboxylase 2 (GAD2) a genetic link between low birth weight and subsequent development of obesity in children? in The Journal of clinical endocrinology and metabolism 2005 (PubMed)

  7. Hamster Polyclonal GAD65 Primary Antibody for IHC, IHC (p) - ABIN4313136 : Lee, Suk, Kang, McGeer, McGeer: Neurotoxic factors released by stimulated human monocytes and THP-1 cells. in Brain research 2011 (PubMed)

  8. Human Monoclonal GAD65 Primary Antibody for IHC (p), IP - ABIN2473839 : Hampe, Lundgren, Daniels, Hammerle, Marcovina, Lernmark: A novel monoclonal antibody specific for the N-terminal end of GAD65. in Journal of neuroimmunology 2001 (PubMed)

More Antibodies against GAD65 Interaction Partners

Rhesus Monkey Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. Results describe the gross distribution patterns of GAD65 in human and rhesus monkey thalami and show that they are nearly identical.

Zebrafish Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. GAD65 mRNA was found in the periaqueductal gray.

Human Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. This study showed a significant 58% increase of SCN (show SRI Antibodies) GAD65/67-ir and a significant 169% increase of SCN (show SRI Antibodies) GAD67 (show GAD1 Antibodies)-mRNA in the depression group.

  2. healthy donor NK cells showed similar degranulation against both GAD65 AA 114-122 pulsed and unpulsed APCs (show APCS Antibodies). The pathogenetic significance of the CD3 (show CD3 Antibodies)-CD8dullCD56+ 'memory-like NK cell subset' with increased response upon secondary challenge in diabetics remains to be elucidated

  3. Genetic variability in GAD2 and GAD1 (show GAD1 Antibodies) contributes to risk of methamphetamine dependence in the Thai population.

  4. Study propose that aberrant accumulation of immunogenic GAD65 in Golgi membranes facilitates inappropriate presentation to the immune system after release from stressed and/or damaged beta-cells, triggering autoimmunity.

  5. UV exposed hydrogen peroxide produces hydroxyl radical that may cause protein damage (GAD-65) to the extent of generating neo-epitopes on the molecule, making it immunogenic.

  6. we investigated the relationship between serum glutamic acid decarboxylase (GAD) autoantibody (Ab) levels and single nucleotide polymorphisms (SNPs) of the glutamic acid de-carboxylase 2 (GAD2) 5'-untranslated region

  7. GAD (show GAD1 Antibodies) autoantibodies were associated with risk of developing type 1 diabetes.

  8. Kaplan-Meier survival curves estimated a worst pancreas graft survival for patients with positive IA-2 (show PTPRN Antibodies) antibodies versus those patients with negative auto-antibodies and GAD65+ auto-antibodies after simultaneous pancreas kidney transplantation.

  9. Plasma GAD65 qualifies as a marker for early beta-cell loss after intraportal transplantation.

  10. Cohort study shows that mean GAD65 mRNA levels in the prefrontal cortex area 9 are not altered in subjects with schizophrenia but are lower in subjects with schizoaffective disorder

Mouse (Murine) Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) interaction partners

  1. These results thus identify a molecularly distinct, orexin (show OX Antibodies)-activated lateral hypothalamus submodule that governs physical activity in mice.

  2. Results suggest age-dependent decrease of GAD65/67 mRNAs but normal densities of certain GABAergic interneurons in the Shank3 (show SHANK3 Antibodies) transgenic mice.

  3. GABA is the major inhibitory neurotransmitter in the brain. We show that Dlx1/Dlx2 homeobox (show PRRX1 Antibodies) genes regulate GABA synthesis during forebrain development through direct activation of glutamic acid decarboxylase enzyme isoforms that convert glutamate (show GRIN1 Antibodies) to GABA.

  4. Treadmill running prevented partial sciatic nerve ligation-induced reductions in GAD65/67 production, and, thus, GABA levels may be retained in interneurons and neuropils in the superficial dorsal horn.

  5. Increased activity due to bicuculline preferentially up-regulated GAD65. This was reduced by AP5, a selective blocker of Ca2 (show CA2 Antibodies)+-permeable ion channels and NMDA-Rs, and by U0126, KN93, and K252a, affecting BDNF (show BDNF Antibodies) pathways.

  6. Data show that miRNAs are involved in regulating the expression of the major type 1 diabetes (T1D) autoantigens IA-2 (show PTPRN Antibodies), IA-2beta (show PTPRN2 Antibodies), and GAD65 enzyme.

  7. Results indicate the significant contribution of not only GAD65, GAD67 (show GAD1 Antibodies) and VGAT (show SLC32A1 Antibodies)-mediated GABAergic but also glycinergic transmissions to both palate and abdominal wall formations

  8. Analysis of GAD65/67 single and double knock-out embryos revealed that the two GADs play complementary (inhibitory) roles in GnRH migration ultimately modulating the speed and/or direction of GnRH migration.

  9. Results show that GAD65 is required for efficient GABAergic synaptic transmission and plasticity, and for maintaining extracellular GABA at (show ABAT Antibodies) a level needed for associative plasticity at cortical inputs in the lateral amygdala

  10. Menin regulates spinal glutamate (show GRIN1 Antibodies)-GABA balance through GAD65 contributing to neuropathic pain.

GAD65 (GAD2) Antigen Profile

Protein Summary

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Gene names and symbols associated with GAD2

  • glutamate decarboxylase 2 (GAD2) antibody
  • glutamate decarboxylase 2 (PTRG_06148) antibody
  • glutamate decarboxylase 2 (LOC9324534) antibody
  • glutamate decarboxylase 2 (Gad2) antibody
  • glutamate decarboxylase 1 (GAD1) antibody
  • glutamate decarboxylase 2 (gad2) antibody
  • glutamic acid decarboxylase 2 (Gad2) antibody
  • 6330404F12Rik antibody
  • F12P19.12 antibody
  • F12P19_12 antibody
  • GAD(65) antibody
  • Gad-2 antibody
  • gad65 antibody
  • glutamate decarboxylase 2 antibody
  • zgc:112198 antibody

Protein level used designations for GAD2

glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) , glutamate decarboxylase 2 , glutamic acid decarboxylase , 65 kDa glutamic acid decarboxylase , GAD-65 , Glutamate decarboxylase 2 (islet) , glutamate decarboxylase 65 kDa isoform , glutamic acid decarboxylase 2 , glutamic acid decarboxylase 65 , Glutamate decarboxylase-2 (pancreas)

GENE ID SPECIES
466026 Pan troglodytes
512459 Bos taurus
613029 Macaca mulatta
4836947 Scheffersomyces stipitis CBS 6054
6344405 Pyrenophora tritici-repentis Pt-1C-BFP
9324534 Arabidopsis lyrata subsp. lyrata
24380 Rattus norvegicus
100344727 Oryctolagus cuniculus
842908 Arabidopsis thaliana
550403 Danio rerio
2572 Homo sapiens
487107 Canis lupus familiaris
395395 Gallus gallus
396929 Sus scrofa
14417 Mus musculus
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