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GAD65 antibody (AA 6-99)

GAD2 Reactivity: Human IHC Host: Mouse Monoclonal MSVA-602M unconjugated
Catalog No. ABIN7477477
  • Target See all GAD65 (GAD2) Antibodies
    GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
    Binding Specificity
    • 16
    • 10
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 6-99
    Reactivity
    • 92
    • 67
    • 40
    • 16
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 92
    • 25
    • 1
    Mouse
    Clonality
    • 95
    • 23
    Monoclonal
    Conjugate
    • 60
    • 13
    • 12
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This GAD65 antibody is un-conjugated
    Application
    • 101
    • 42
    • 40
    • 21
    • 17
    • 17
    • 13
    • 13
    • 13
    • 13
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)
    Clone
    MSVA-602M
    Isotype
    IgG2b, kappa
    Top Product
    Discover our top product GAD2 Primary Antibody
  • Application Notes
    IHC 1:100-1:200
    Comment

    Positive Control: Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells.

    Negative Control: Colon: GAD2 immunostaining should be absent in all cell types

    Protocol
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
    Alternative Name
    GAD2 (GAD2 Products)
    Synonyms
    GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody
    Background
    GAD65,GAD2 antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    Q05329
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