GAD65 antibody (AA 6-99)
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- Target See all GAD65 (GAD2) Antibodies
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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Binding Specificity
- AA 6-99
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GAD65 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)
- Clone
- MSVA-602M
- Isotype
- IgG2b, kappa
- Top Product
- Discover our top product GAD2 Primary Antibody
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- Application Notes
- IHC 1:100-1:200
- Comment
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Positive Control: Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells.
Negative Control: Colon: GAD2 immunostaining should be absent in all cell types
- Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Alternative Name
- GAD2 (GAD2 Products)
- Synonyms
- GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody
- Background
- GAD65,GAD2 antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- Q05329
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