GAD65 antibody (AA 6-99)

Catalog No. ABIN6939483

The Mouse Monoclonal anti-GAD65 antibody (Clone GAD2-1960) (ABIN6939483) specifically detects GAD65 in IHC. The antibody is reactive with Human samples.

Quick Overview for GAD65 antibody (AA 6-99) (ABIN6939483)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: GAD2-1960

Product Details anti-GAD65 Antibody

Binding Specificity: AA 6-99

Purpose: GAD2 / GAD65 (GABAergic Neuronal Marker) Antibody

Specificity: This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes:

Positive Control: Pancreas or Brain (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background:

DCE2, GAD2, Glutamic Acid Decarboxylase 2 (Pancreas), Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65 kDa), Glutamic Acid Decarboxylase 65 (GAD65)

Pancreas or Brain (IHC).

Molecular Weight: 65kDa

Gene ID: 2572, 231829

UniProt: Q05329