CYB5R4 antibody (AA 444-458)

Catalog No. ABIN1019669

The Goat Polyclonal anti-CYB5R4 antibody has been validated for WB and ELISA. It is suitable to detect CYB5R4 in samples from Rat.

Quick Overview for CYB5R4 antibody (AA 444-458) (ABIN1019669)

Target: CYB5R4 (Cytochrome B5 Reductase 4 (CYB5R4))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CYB5R4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CYB5R4 Antibody

Binding Specificity: AA 444-458

Purpose: Cyb5r4 (mouse aa444-458)

Sequence: RCQLEKLALR EKRFD

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence RCQLEKLALREKRFD, from the internal region of the protein sequence according to NP_077157.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65 kDa band observed in Rat Spleen lysates (calculated MW of 55 kDa according to NP_596918.2 and 58.8 kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYB5R4

Target: CYB5R4 (Cytochrome B5 Reductase 4 (CYB5R4))

Alternative Name: Cyb5r4

Background: Cyb5r4, cytochrome b5 reductase 4, 2810034J18Rik, B5+B5R, C79736, Cb5cb5r, Ncb5or, b5/b5r, b5b5r, N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein, NADPH cytochrome B5 oxidoreductase, cb5/cb5R, cytochrome b-type NAD(P)H

Gene ID: 266690

NCBI Accession: NP_077157

Pathways: Hormone Transport, Carbohydrate Homeostasis