Vimentin antibody (HRP)

Catalog No. ABIN1042494

This anti-Vimentin antibody is a Mouse Monoclonal antibody detecting Vimentin in WB, FACS, IHC (p), ICC and IHC (fro). Suitable for Human, Mouse, Rat, Pig, Chicken, Monkey, Dog, Goat, Hamster and Zebrafish (Danio rerio). This Primary Antibody has been cited in 5+ publications.

Quick Overview for Vimentin antibody (HRP) (ABIN1042494)

Target: Vimentin (VIM)

Reactivity: Human, Mouse, Rat, Pig, Chicken, Monkey, Dog, Goat, Hamster, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is conjugated to HRP

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RV202

Product Details anti-Vimentin Antibody (HRP)

Specificity: RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:10.

Characteristics: Cytoskeletal,Tumour marker

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Comment:

RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0,1% BSA, 0.09% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Vimentin antibody (HRP) (ABIN1042494)

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Pathways: Caspase Cascade in Apoptosis