CYBB antibody

Catalog No. ABIN1042596

This anti-CYBB antibody is a Mouse Monoclonal antibody detecting CYBB in WB, IF, IHC (p) and ICC. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CYBB antibody (ABIN1042596)

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: 54-1

Product Details anti-CYBB Antibody

Specificity: This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389

Purification: Protein G

Immunogen: Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)

Isotype: IgG1

Application Details

Application Notes: Optimal antibody concentration should be determined by titration, however as a guideline try,WB: Use at an assay dependent dilution. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).ICC/IF: Use a concentration of 1 μg/mL.IHC-P: Use a concentration of 5 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-CYBB antibody (ABIN1042596)

Baniulis, Nakano, Nauseef, Banfi, Cheng, Lambeth, Burritt, Taylor, Jesaitis: "Evaluation of two anti-gp91phox antibodies as immunoprobes for Nox family proteins: mAb 54.1 recognizes recombinant full-length Nox2, Nox3 and the C-terminal domains of Nox1-4 and cross-reacts with GRP 58." in: Biochimica et biophysica acta, Vol. 1752, Issue 2, pp. 186-96, (2005) (PubMed).

Burritt, Quinn, Jutila, Bond, Jesaitis: "Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16974-80, (1995) (PubMed).

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: NOX2/gp91phox

Background: Synonyms:
CGD91-phox antibody,CYBB antibody,Cytochrome b(558) beta chain antibody,Cytochrome b-245 heavychain antibody,gp91-1 antibody,gp91-phox antibody,Heme-binding membrane glycoprotein gp91phox antibody,NADPHoxidase 2 antibody,Neutrophil cytochrome b 91 kDa polypeptide antibody,NOX2 antibody,p22 phagocyte B-cytochromeantibody,Superoxide-generating NADPH oxidase heavy chain subunit antibody

Gene ID: 21577

OMIM: 300481

UniProt: Q01062