CYBB antibody (AA 416-500)

Catalog No. ABIN5692996

Product Details anti-CYBB Antibody

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Binding Specificity: AA 416-500

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYBB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NOX2/gp91phox detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-CYBB antibody (ABIN5692996)

Zhang, Deng, Lai, Guan, Sun, Han, Wang, Pan, Ji, Luo, Huang, Tang, Gu, Dan, Yu, Namaka, Zhang, Deng, Li: "Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation." in: Scientific reports, Vol. 6, pp. 30146, (2018) (PubMed).

Deep, Kumar, Jain, Dhar, Panigrahi, Hussain, Agarwal, El-Elimat, Sica, Oberlies, Agarwal: "Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity." in: Scientific reports, Vol. 6, pp. 23135, (2017) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." in: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Du, Yang, Zhou, Liu, Li, Chen, Gao: "D?galactose?induced mitochondrial DNA oxidative damage in the auditory cortex of rats." in: Molecular medicine reports, Vol. 10, Issue 6, pp. 2861-7, (2014) (PubMed).

Target Details for CYBB

Target: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Alternative Name: CYBB (CYBB Products)

Synonyms: zgc:64144 antibody, cgd antibody, gp91-1 antibody, gp91-phox antibody, gp91phox antibody, nox2 antibody, NOX2 antibody, cybb antibody, NOX1 antibody, AMCBX2 antibody, CGD antibody, GP91-1 antibody, GP91-PHOX antibody, GP91PHOX antibody, p91-PHOX antibody, Nox2 antibody, C88302 antibody, CGD91-phox antibody, Cgd antibody, Cyd antibody, Gp91-phox antibody, CYBB antibody, cytochrome b-245, beta polypeptide (chronic granulomatous disease) antibody, cytochrome b-245, beta polypeptide antibody, cytochrome b-245 heavy chain antibody, cytochrome b-245, beta polypeptide L homeolog antibody, cytochrome b-245 beta polypeptide antibody, cytochrome b-245 beta chain antibody, NADPH oxidase heavy chain subunit antibody, cybb antibody, CYBB antibody, cybb.L antibody, Cybb antibody, GP91-PHOX antibody

Background:

Synonyms: Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, CYBB, NOX2

Tissue Specificity: Detected in neutrophils (at protein level).

Background: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

UniProt: P04839