CYBA antibody

Catalog No. ABIN1042639

This Mouse Monoclonal antibody specifically detects CYBA in WB. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for CYBA antibody (ABIN1042639)

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CYBA antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 44-1

Product Details anti-CYBA Antibody

Specificity: This antibody is specific for Human Cytochrome b245 Light Chain 29-TAGRF-33 & 183-PQVNPI-188

Purification: Protein G purified

Immunogen: Tissue / cell preparation (Human): Partially Purified human neutrophil flavocytochrome b (heparin Ultrogel/lectin affinity purification - in Triton X-100)

Isotype: IgG2

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try,WB: Use at 1:1000. Predicted molecular weight: 21 kDa

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-CYBA antibody (ABIN1042639)

Taylor, Jesaitis: "Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics." in: Methods in molecular biology (Clifton, N.J.), Vol. 412, pp. 429-37, (2008) (PubMed).

Burritt, Busse, Gizachew, Siemsen, Quinn, Bond, Dratz, Jesaitis: "Antibody imprint of a membrane protein surface. Phagocyte flavocytochrome b." in: The Journal of biological chemistry, Vol. 273, Issue 38, pp. 24847-52, (1998) (PubMed).

Burritt, Quinn, Jutila, Bond, Jesaitis: "Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries." in: The Journal of biological chemistry, Vol. 270, Issue 28, pp. 16974-80, (1995) (PubMed).

Target Details for CYBA

Target: CYBA (Cytochrome B-245, alpha Polypeptide (CYBA))

Alternative Name: Cytochrome b245 Light Chain 

Background: Synonyms:
Superoxide-generating NADPH oxidase light chain subunit antibody,CYBA antibody,Cytochrome b(558) alphachain antibody,Cytochrome b558 subunit alpha antibody,Neutrophil cytochrome b 22 kDa polypeptide antibody,p22phagocyte B-cytochrome antibody,p22-phox antibody,p22phox antibody

Gene ID: 1535, 21577

OMIM: 608508

UniProt: Q01062

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis