CASR antibody (N-Term)

Catalog No. ABIN1048374

Product Details anti-CASR Antibody

Target: CASR (Calcium-Sensing Receptor (CASR))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey, Rabbit, Chicken, Dog, Hamster, Horse, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CASR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Xenopus (94%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from N-terminal extracellular domain of human CASR. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Xenopus (94%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (4 - 8 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CASR

Target: CASR (Calcium-Sensing Receptor (CASR))

Alternative Name: CASR/Calcium Sensing Receptor (CASR Products)

Synonyms: CAR antibody, EIG8 antibody, FHH antibody, FIH antibody, GPRC2A antibody, HHC antibody, HHC1 antibody, HYPOC1 antibody, NSHPT antibody, PCAR1 antibody, CaR antibody, Gprc2a antibody, gprc2a antibody, xV2R F-1 antibody, CASR antibody, calcium sensing receptor antibody, calcium-sensing receptor antibody, calcium-sensing receptor L homeolog antibody, CASR antibody, Casr antibody, casr.L antibody

Background: Name/Gene ID: CASR
Subfamily: Calcium-sensing
Family: GPCR

Synonyms: CASR, Ca sensing receptor, Ca2+ sensing receptor, Calcium-sensing receptor, FIH, EIG8, GPRC2A, HHC, HHC1, Hypocalciuric hypercalcemia 1, PCAR1, NSHPT, CAR, FHH

Gene ID: 846

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis