CRHR1 antibody (Extracellular)

Catalog No. ABIN7043086

This Rabbit Polyclonal antibody specifically detects CRHR1 in WB, IHC, IF, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for CRHR1 antibody (Extracellular) (ABIN7043086)

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRHR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CRHR1 Antibody

Binding Specificity: AA 31-44, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Corticotropin-Releasing Factor Receptor 1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: 14 amino acid residues identical, human - 11,Mouse -12

Characteristics: Anti-CRF1/CRHR1 (extracellular) Antibody (ABIN7043086, ABIN7044185 and ABIN7044186) is a highly specific antibody directed against an extracellular epitope of the rat Corticotropin-releasing factor receptor 1. It can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize CRFR1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235173)

Blocking Peptide: (ABIN7235173)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CRHR1

Target: CRHR1 (Corticotropin Releasing Hormone Receptor 1 (CRHR1))

Alternative Name: CRHR1

Background: Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1,Corticotrophin-releasing factor (CRF) is a peptide hormone and a key regulator of the stress response. Through activation of the hypothalamic-pituitary axis and its role as a neurotransmitter, CRF influences a wide range of physiological responses, including appetite control, cardiovascular regulation, glucose metabolism, immune function and behavior.Corticotrophin-releasing factor receptor 1 (CRHR1, CRF1) is a member of the class B G-protein coupled receptors. CRHR1 is activated by CRF. It is expressed in brain areas including the hypothalamus, pituitary, amygdala and cortex. The core fold of the receptor features seven transmembrane helices (TM1-TM7). The connecting loops lack secondary structure apart from one extracellular loop- ECL1. In contrary to A Class GPCRs, CRF1 has a pronounced V-shape and a large cavity hypothesized to be the peptide binding site. The cytoplasmic half of TM3 and TM5 interacts with Gs. Small molecule antagonists of the receptor, such as CP-376395, act allosterically by maintaining the receptor in its inactive state at a site different from the peptide binding site. Interestingly, although CP-376395 is highly selective for CRHR1, all of the residues found to interact directly with this ligand are conserved between CRHR1 and CRHR21.Interaction between childhood trauma and sequence variation in the CRHR1 gene that increase risk for affective disorders has been established by several studies. In Rhesus macaques, single nucleotide polymorphisms affecting exon 6 of CRHR1 gene influence anxious temperament (a phenotype that predicts the development of human anxiety and depressive disorder) and brain metabolism2.

Alternative names: CRF1 (CRHR1), Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1

Gene ID: 58959

NCBI Accession: NM_001145146

UniProt: P35353

Pathways: Hormone Transport, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Feeding Behaviour, Negative Regulation of Transporter Activity