HUNK antibody (AA 220-300)

Catalog No. ABIN7221478

This Rabbit Polyclonal antibody specifically detects HUNK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HUNK antibody (AA 220-300) (ABIN7221478)

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HUNK Antibody

Binding Specificity: AA 220-300

Purpose: Rabbit Anti-HUNK Polyclonal Antibody

Specificity: HUNK Polyclonal Antibody detects endogenous levels of HUNK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human HUNK at AA range: 220-300

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HUNK

Target: HUNK (Hormonally Up-Regulated Neu-Associated Kinase (HUNK))

Alternative Name: HUNK

Background: HUNK, MAKV, Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-VThe HUNK (hormonally upregulated Neu-associated kinase) protein, also designated MAK-V in mouse, has been identified as a novel SNF1-related serine/threonine kinase. The human HUNK gene localizes to chromosome 21q22 and encodes a protein with nucleocytoplasmic distribution and localizes to the centrosome. Overexpression of the HUNK protein associates with approximately 50 % of breast carcinomas, and may provide diagnostic-prognostic value as a molecular marker. Serine/threonine-protein kinase SNF1-like kinase 2 (SIK) phosphorylates Ser-794 of IRS1 in insulin-stimulated adipocytes, which may modulate the efficiency of insulin signal transduction. SIK is activated by phosphorylation on Thr-175 by STK11 in complex with STE20-related adapter-alpha and CAB39.

Molecular Weight: 75kD

Gene ID: 30811

UniProt: P57058