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Peroxidase antibody

Reactivity: Horseradish ELISA, WB, IHC Host: Guinea Pig Polyclonal unconjugated
Catalog No. ABIN105506
  • Target See all Peroxidase Antibodies
    Peroxidase
    Reactivity
    Horseradish
    Host
    • 2
    • 2
    • 1
    • 1
    Guinea Pig
    Clonality
    • 5
    • 1
    Polyclonal
    Conjugate
    • 6
    This Peroxidase antibody is un-conjugated
    Application
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Cross-Reactivity (Details)
    Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
    Purity
    Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Guinea Pig Serum as well as purified and partially purified Peroxidase [Horseradish].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Peroxidase [Horseradish]
    Immunogen Type: Native Protein
    Isotype
    IgG
    Top Product
    Discover our top product Peroxidase Primary Antibody
  • Application Notes
    Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.
    ELISA Dilution: 1:20.000 - 1:100.000
    Immunohistochemistry Dilution: 1:1.000 - 1:5.000
    Western Blot Dilution: 1:2.000 - 1:10.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Peroxidase
    Abstract
    Peroxidase Products
    Synonyms
    peroxidase antibody, PER1 antibody, ShK domain and PerOxidase domain containing protein antibody, Peroxidase skpo-1 antibody, hypothetical protein antibody, peroxidase 1 antibody, 1-Cys-peroxiredoxine antibody, skpo-3 antibody, skpo-1 antibody, T06D8.10 antibody, C46A5.4 antibody, K10B4.1 antibody, R08F11.7 antibody, WSP1 antibody, PER1 antibody
    Background
    Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Peroxidase (Horseradish) (Guinea Pig) Antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research. Synonyms: HRP, Horseradish peroxidase, Guinea pig-anti-peroxidase antibody
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