Actin, gamma 1 antibody (AA 1-375)

Catalog No. ABIN1077746

Product Details anti-Actin, gamma 1 Antibody

Target: Actin, gamma 1 (ACTG1)

Binding Specificity: AA 1-375

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: MGHHHHHHSG SEF-MEEEIAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF

Specificity: It has been selected for its ability to recognize ACTg1 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant ACTg1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against ACTg1.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant ACTg1 (Met1~Phe375) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for Actin, gamma 1

Target: Actin, gamma 1 (ACTG1)

Alternative Name: Actin Gamma 1 (ACTg1) (ACTG1 Products)

Synonyms: ACT antibody, ACTG antibody, BRWS2 antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, ACTA1 antibody, actg1a antibody, Actg antibody, actg1b antibody, AL023024 antibody, Actl antibody, E51 antibody, actin antibody, cytoplasmic antibody, actin gamma 1 antibody, actin, gamma 1 a antibody, actin, gamma 1 antibody, actin gamma 1 S homeolog antibody, actin, gamma, cytoplasmic 1 antibody, Actin, gamma 1 antibody, ACTG1 antibody, actg1 antibody, Actg1 antibody, actg1.S antibody

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization