Cytochrome C antibody (AA 5-100)

Catalog No. ABIN1077973

This Rabbit Polyclonal antibody specifically detects Cytochrome C in WB, IHC, IP and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Cytochrome C antibody (AA 5-100) (ABIN1077973)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Cytochrome C Antibody

Binding Specificity: AA 5-100

Purpose: Polyclonal Antibody to Cytochrome C (CYCS)

Specificity: The antibody is a rabbit polyclonal antibody raised against CYCS. It has been selected for its ability to recognize CYCS in immunohistochemical staining and western blotting.

Cross-Reactivity: Chicken, Cow, Dog, Goat, Guinea Pig, Horse, Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cytochrome C (CYCS) corresdonding to Glu5~Lys100 (Accession # P99999)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-5 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-Cytochrome C antibody (ABIN1077973)

Ding, Han, Chen, Fang, Liu, Wang, Wei, Kodithuwakku, Li: "Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway." in: Molecules (Basel, Switzerland), Vol. 21, Issue 6, (2016) (PubMed).

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: CYCS

Background: Cyt-C, HCS, CYC, CytC

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity