Cyt F antibody

Catalog No. ABIN108466

This Rabbit Polyclonal antibody specifically detects Cyt F in WB. It exhibits reactivity toward Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum, Barley, Ulva prolifera, Synechocystis, Selaginella martensii, Nostoc (Cyanobacteria), Nannochloropsis gaditana, Craterostigma pumilum, Colobanthus quitensis, Chlorella vulgaris and Physcomytrium Patens. It has been mentioned in 4+ publications

Quick Overview for Cyt F antibody (ABIN108466)

Target: Cyt F

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum, Barley, Ulva prolifera, Synechocystis, Selaginella martensii, Nostoc (Cyanobacteria), Nannochloropsis gaditana, Craterostigma pumilum, Colobanthus quitensis, Chlorella vulgaris, Physcomytrium Patens

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

Cross-Reactivity (Details): Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella vulgaris, Colobanthus quitensis Kunt Bartl, CRaterostigma pumilum, Hordeum vulgare, Nannochloropsis gaditana, Nicotiana tabacum, Nostoc sp. PCC7120, Physcomitrium patens, Selaginella martensi, Synechocystis sp. 6803 substrain PCC-M, Ulva prolifera

Predicted Reactivity: Algae,Citrus x limon,Zea mays

Purification: Serum

Immunogen: GST fusion to cytochrome f from Chlamydomonas reinhartii P23577

Application Details

Application Notes: 1 : 2000-1 : 50 000 (WB)

Comment:

reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Charuvi, Nevo, Shimoni, Naveh, Zia, Adam, Farrant, Kirchhoff, Reich: "Photoprotection conferred by changes in photosynthetic protein levels and organization during dehydration of a homoiochlorophyllous resurrection plant." in: Plant physiology, Vol. 167, Issue 4, pp. 1554-65, (2015) (PubMed).

Simionato, Block, La Rocca, Jouhet, Maréchal, Finazzi, Morosinotto: "The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus." in: Eukaryotic cell, Vol. 12, Issue 5, pp. 665-76, (2013) (PubMed).

Heinnickel, Alric, Wittkopp, Yang, Catalanotti, Dent, Niyogi, Wollman, Grossman: "Novel thylakoid membrane GreenCut protein CPLD38 impacts accumulation of the cytochrome b6f complex and associated regulatory processes." in: The Journal of biological chemistry, Vol. 288, Issue 10, pp. 7024-36, (2013) (PubMed).

Lang, Mueller, Hoernstein, Porankiewicz-Asplund, Vervliet-Scheebaum, Reski: "Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics." in: Plant cell reports, Vol. 30, Issue 2, pp. 205-15, (2011) (PubMed).

Target Details

Target: Cyt F

Background:

Background: Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c'-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Additional information: Contains 0.02 % sodium azide as preservative

Molecular Weight: 34 | 31-32 kDa

Gene ID: 2716989

UniProt: P23577