Cyt F antibody

Catalog No. ABIN7833205

The Rabbit Polyclonal anti-Cyt F antibody is suitable to detect Cyt F in samples from Chlamydomonas reinhardtii. It has been validated for WB.

Quick Overview for Cyt F antibody (ABIN7833205)

Target: Cyt F

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex (algal)

Cross-Reactivity (Details): Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Euglena gracilis,Chlorella vulgaris,Synechocystis sp.,Nostoc sp.,Prochlorococcus marinus subsp. Pastoris,Synechococcus sp.,Prochlorococcus marinus,Synechococcus elongatus

Purification: Antigen affinity purified serum

Immunogen: KLH-conjugated peptide derived from Chlamydomonas reinhardtii Cytf (PetA)

Application Details

Application Notes: 1 : 10 000 - 1: 15 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution, add 50 μL, of sterile or deionized water.

Buffer: Antigen affinity purified serum, in PBS pH 7.4

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details

Target: Cyt F

Background: Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c'-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Molecular Weight: 34 kDa

UniProt: P23577