KEAP1 antibody (AA 380-624)

Catalog No. ABIN1098137

The Mouse Monoclonal anti-KEAP1 antibody has been validated for ELISA, ICC and FACS. It is suitable to detect KEAP1 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for KEAP1 antibody (AA 380-624) (ABIN1098137)

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KEAP1 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1F10B6

Product Details anti-KEAP1 Antibody

Binding Specificity: AA 380-624

Purpose: KEAP1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-KEAP1 antibody (ABIN1098137)

Kim, You, Lee, Ahn, Seong, Hwang: "Suppression of NF-kappaB signaling by KEAP1 regulation of IKKbeta activity through autophagic degradation and inhibition of phosphorylation." in: Cellular signalling, Vol. 22, Issue 11, pp. 1645-54, (2010) (PubMed).

Zhang, Singh, Yegnasubramanian, Esopi, Kombairaju, Bodas, Wu, Bova, Biswal: "Loss of Kelch-like ECH-associated protein 1 function in prostate cancer cells causes chemoresistance and radioresistance and promotes tumor growth." in: Molecular cancer therapeutics, Vol. 9, Issue 2, pp. 336-46, (2010) (PubMed).

Target Details for KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Alternative Name: KEAP1

Background: This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.

Molecular Weight: 69.7 kDa

Gene ID: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location