GDF15 antibody (C-Term) (HRP)

Catalog No. ABIN1101715

This Rabbit Polyclonal antibody specifically detects GDF15 in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for GDF15 antibody (C-Term) (HRP) (ABIN1101715)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF15 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GDF15 Antibody (HRP)

Binding Specificity: C-Term

Specificity: This antibody reacts with the C-terminus of endogenous NAG-1 protein from human and mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100 % homology. Partial reactivity is expected against rat based on an 86 % homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.

Purification: Immunoaffinity purified

Immunogen: Anti-NAG-1 (C-terminal specific) antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC (1:500 - 1:2500), WB (1:1000 - 1:5000)

Usage: Anti-NAG-1 (C-terminal specific) antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of approximately 14 and 28 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15

Background: Name/Gene ID: GDF15
Family: TGF beta

Synonyms: GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated gene 1 protein, PLAB, NSAID-activated gene 1 protein, Placental TGF-beta, PTGFB, GDF-15, PTGF-beta

Gene ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome