IL-2 antibody

Catalog No. ABIN1102474

This Goat Polyclonal antibody specifically detects IL-2 in WB, IHC, ELISA, Neut and IHC (p). It exhibits reactivity toward Human.

Quick Overview for IL-2 antibody (ABIN1102474)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IL-2 Antibody

Specificity: Human IL-2

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-2.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), Neut (0.12 - 0.16 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: LS-B7472 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-2 (2 ng/mL), a concentration of 0.12-0.16 μg/mL of this antibody is required. ELISA: To detect hIL-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-2 (LS-C104359) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-2. Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2

Background: Name/Gene ID: IL2
Family: Interleukin

Synonyms: IL2, Aldesleukin, Interleukin 2, Lymphokine, IL-2, Interleukin-2, TCGF, T cell growth factor, T-cell growth factor

Gene ID: 3558

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation