Resistin antibody

Catalog No. ABIN1103967

The Goat Polyclonal anti-Resistin antibody (ABIN1103967) specifically detects Resistin in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Resistin antibody (ABIN1103967)

Target: Resistin (RETN)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Resistin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Resistin Antibody

Specificity: Human Resistin

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Resistin.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7427 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Resistin (LS-C104464) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hResistin. Western Blot: To detect hResistin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hResistin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Resistin

Target: Resistin (RETN)

Alternative Name: RETN / Resistin

Background: Name/Gene ID: RETN

Synonyms: RETN, ADSF, Fizz3, Resistin, RETN1, Xcp1, RSTN, Found in inflammatory zone 3, HXCP1, Resistin delta2

Gene ID: 56729

UniProt: Q9HD89

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration