CD25 antibody (Biotin)

Catalog No. ABIN1106144

The Mouse Monoclonal anti-CD25 antibody is suitable to detect CD25 in samples from Human. It has been validated for FACS.

Quick Overview for CD25 antibody (Biotin) (ABIN1106144)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: B-B10

Product Details anti-CD25 Antibody (Biotin)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ion Exchange Chromatography.

Immunogen: PHA activated T cells. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.0 mL deionised water.

Buffer: PBS, 0.09 % Sodium Azide, 1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted after reconstitution at 2-8 °C.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25 / IL2RA

Background: IL-2R (T-cell growth factor receptor) consists of a chain of 55 kDa (also known as Tac antigen or p55 or alpha chain) and a p75 or beta chain. IL-2RA is over-expressed on the surface of activated Tcells, HTLV-1 or -2 positive T-cell lines, and moderately expressed on activated B-cells, NK-cells, and macrophages. CD25 Molecule alone appears to function as a low affinity receptor and associates wtih CD122 (IL-2R beta chain, p75) and CD132 (common gamma chain) to form the high affinity IL-2 receptor complex.Synonyms: IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, Interleukin-2 receptor alpha chain, TAC antigen, p55

Gene ID: 3559

NCBI Accession: NP_000408

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation