CDK2 antibody

Catalog No. ABIN1106646

The Mouse Monoclonal anti-CDK2 antibody (Clone 8A12) (ABIN1106646) specifically detects CDK2 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CDK2 antibody (ABIN1106646)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 8A12

Product Details anti-CDK2 Antibody

Specificity: This antibody recognizes human Cdk2 and human Cdk5 and does not recognize human Cdc2 (Cdk1) on Western blot.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Protein A agarose

Immunogen: Recombinant human Cdk2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Prepare a stock solution by dissolving the lyophilized antibody 100 μL of distilled water. After reconstitution, the IgG concentration should be 1 mg/mL.

Buffer: PBS ( pH 7.2) containing 1 % sucrose, 0.09 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Background: Cellular proliferation is controlled by a family of protein kinases in which the catalytic subunits are members of the cyclin-dependent kinase (cdk) family and the regulatory subunits are cyclins. To date, nine distinct Cdks in addition to eight different cyclins have been identified, in which different CDK/cyclin combinations serve to regulate distinct points in the mammalian cell division cycle. Although Cdc2Hs (Cdk1)/cyclin B controls the transition of cells from the G2 to M-phase, the activities of Cdk2/cyclin E and Cdk2/cyclin A are critical for G1/S-phase transition and progression through S-phase.Synonyms: Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase

Gene ID: 1017

NCBI Accession: NP_001789

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA