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CKB antibody (N-Term)

This anti-CKB antibody is a Mouse Monoclonal antibody detecting CKB in WB, IF, IHC (fro) and IHC (p). Suitable for Human, Mouse, Rat and Rabbit.
Catalog No. ABIN1106703

Quick Overview for CKB antibody (N-Term) (ABIN1106703)

Target

See all CKB Antibodies
CKB (Creatine Kinase, Brain (CKB))

Reactivity

  • 111
  • 44
  • 37
  • 16
  • 6
  • 6
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Mouse, Rat, Rabbit

Host

  • 86
  • 57
  • 1
Mouse

Clonality

  • 74
  • 69
Monoclonal

Conjugate

  • 87
  • 21
  • 10
  • 4
  • 4
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CKB antibody is un-conjugated

Application

  • 100
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  • 50
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  • 19
  • 16
  • 7
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  • 2
  • 2
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  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

CK-BYK-21E10
  • Binding Specificity

    • 25
    • 8
    • 8
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    N-Term

    Sequence

    SNSHNALKLR FPAEDEF

    Specificity

    The monoclonal antibody CK-BYK/21E10 recognizes creatine kinase B-type, also known as CKB.

    Cross-Reactivity (Details)

    Species reactivity (tested):Human, mouse, rat, rabbit

    Purification

    Protein G

    Immunogen

    Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain-type

    Isotype

    IgG2b
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    0.1 mg/mL

    Buffer

    PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store at 2 - 8 °C.
  • Target

    CKB (Creatine Kinase, Brain (CKB))

    Alternative Name

    CKBB

    Background

    Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development.Synonyms: B-CK, CKB, Creatine kinase B chain, Creatine kinase B-type, Creatine kinase BB

    Gene ID

    1152

    NCBI Accession

    NP_001814

    UniProt

    P12277
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