Complement C4A/C4B (Chain alpha 2) antibody

Catalog No. ABIN1106789

The Mouse Monoclonal anti- antibody has been validated for WB, EIA and IHC (fro). It is suitable to detect in samples from Human, Cow, Monkey and Pig.

Quick Overview for Complement C4A/C4B (Chain alpha 2) antibody (ABIN1106789)

Target: Complement C4A/C4B

Reactivity: Human, Cow, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 12D11

Product Details

Binding Specificity: Chain alpha 2

Specificity: The monoclonal antibody 12D11 (also known as clone M4d3) recognizes an epitope on the alpha 2 chain of human C4d. The antibody 12D11 recognizes C4, C4b and C4d.

Cross-Reactivity (Details): Species reactivity (tested):Human, Baboon, Rhesus monkey, Bovine, Swine.

Purification: Protein G

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
Western blot: The typical starting working dilution is 1:50
Immunoassay

Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % Sodium Azide, 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details

Target: Complement C4A/C4B

Background: Complement factor C4 (MW 41 kDa), formerly known as Gg protein, consists of an alpha-, beta- and gamma-chain. The classical pathway of complement and the mannose binding lectin (MBL) activation pathway converge at C4. Activated C1, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. The latter can be cleaved by factor I resulting in C4c and C4d, in which step all functional sites are lost. The C4d activation fragment of C4 is an excellent marker for classical complement pathway and MBL pathway activation. The thioester formed between the side chains of Cys1010 and Gln1013 within the C4d region of the α-chain mediates covalent attachment to the target surface bearing activated forms of C1s or MASP. Furthermore, C4d is highly homologous to C3d with over 35 % shared amino acid sequence. In a number of diseases such as rheumatoid arthritis (RA), hereditary angioedema (HAE), systemic lupus erythematosus (SLE) and chronic urticaria with hypercomplementemia levels of C4d are significantly elevated in serum or plasma. C4d levels may also be elevated in plasma from patients with a variety of humoral autoimmune diseases in which complement activation is known to occur. Deposition of C4d in peritubular capillaries has been shown to be a sensitive marker for antibody-mediated (humoral) rejection in renal transplant biopsies.Synonyms: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4

Gene ID: 720

NCBI Accession: NP_001239133

UniProt: P0C0L4