PDGFRA antibody (Extracellular Domain)

Catalog No. ABIN110689

This Mouse Monoclonal antibody specifically detects PDGFRA in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for PDGFRA antibody (Extracellular Domain) (ABIN110689)

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFRA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 57-20 (35248)

Product Details anti-PDGFRA Antibody

Binding Specificity: Extracellular Domain

Specificity: This antibody detects PDGF RECEPTOR ALPHA. The ND50 was determined as 0.2-0.6 μg/mL for blocking 10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of Human WS1 cells.

Purification: Affinity Chromatography on Protein G

Immunogen: Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Isotype: IgG1

Application Details

Application Notes: ELISA: 0.5-1 μg/mL. Western Blot: 1-2 μg/mL. Immunohistochemistry on paraffin embedded sections: 10-20 μg/mL. Functional assays: The ND50 for this batch was determined as 0.2-0.6 μg/mL for blocking10 ng/mL of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 10 % Trehalose as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid multiple freeze/thaw cycles.

Target Details for PDGFRA

Target: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Alternative Name: CD140a / PDGFRA

Background: PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cellsurface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha

Gene ID: 5156

NCBI Accession: NP_006197

UniProt: P16234

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling