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Histone H2B antibody

This Mouse Monoclonal antibody specifically detects Histone H2B in WB, IF and IP. It exhibits reactivity toward Human, Mouse, Rat and Hamster.
Catalog No. ABIN1107547

Quick Overview for Histone H2B antibody (ABIN1107547)

Target

See all Histone H2B Antibodies
Histone H2B

Reactivity

  • 210
  • 97
  • 86
  • 13
  • 10
  • 5
  • 5
  • 5
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
Human, Mouse, Rat, Hamster

Host

  • 218
  • 21
  • 2
Mouse

Clonality

  • 209
  • 32
Monoclonal

Conjugate

  • 164
  • 12
  • 8
  • 7
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This Histone H2B antibody is un-conjugated

Application

  • 167
  • 96
  • 54
  • 45
  • 34
  • 28
  • 27
  • 26
  • 21
  • 16
  • 7
  • 6
  • 6
  • 4
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone

C14264
  • Cross-Reactivity (Details)

    Species reactivity (tested):Human, mouse, rat, hamster

    Purification

    Protein A agarose

    Immunogen

    Protein fraction extracted from the mitotic chromosome

    Isotype

    IgG2b
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS containing 50 % glycerol, pH 7.2, without preservatives

    Preservative

    Without preservative

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Store (in aliquots) at -20 °C.
  • Target

    Histone H2B

    Background

    The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H2B was suppressed to greater extent than that of the others, with histone H3 being relatively least affected. The analysis of acetylation status of each of the four lysine sites at the N-terminal tail of histone H2B revealed decreases consistent with those observed in the total acetylation patterns, with the K12 and K20 residues being markedly more affected than K5 and K15 residues.Synonyms: H2B Histone Family, HIST1H2B

    Molecular Weight

    14 kDa
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