IDO1 antibody

Catalog No. ABIN1107630

This anti-IDO1 antibody is a Sheep Polyclonal antibody detecting IDO1 in WB, IF, IHC (p), FACS and EIA. Suitable for Human.

Quick Overview for IDO1 antibody (ABIN1107630)

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details anti-IDO1 Antibody

Specificity: The polyclonal antibody recognizes human indoleamine 2,3-dioxygenase (IDO). Reactivity of the polyclonal antibody with IDO has been confirmed on immuno blot with IFN-gamma stimulated human peripheral blood lymphocytes.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A

Immunogen: Recombinant IDO

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO1 / INDO

Background: IDO is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules, like tryptophan, serotonin and melatonin. By doing this, IDO initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO induction. The production of IFN-gamma and induction of IDO represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia. By local depletion of tryptophan and increasing proapoptotic kynurenines, IDO greatly affects T-cell proliferation and survival, both in vitro and in vivo, and also affects B-cell and NK-cell function and survival. There is a central role for IDO expression in tolerance involving regulatory cells and DCs. IDO acts as an intermediate pathway in LPS-induced production of reactive oxygen species and NF-kappaB activation, two processes that lead to DC maturation.Synonyms: 3-dioxygenase, 3-dioxygenase 1, EC=1.13.11.52, IDO, IDO1, INDO, Indoleamine 2, Indoleamine-pyrrole 2

Gene ID: 3620

NCBI Accession: NP_002155

UniProt: P14902

Pathways: Activated T Cell Proliferation