IDO1 antibody

Catalog No. ABIN1169195

Product Details anti-IDO1 Antibody

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Recognizes mouse IDO. Detects a band of ~45 kDa by Western blot.

Cross-Reactivity: Mouse (Murine)

Sterility: 0.2 μm filtered

Immunogen: Recombinant mouse IDO.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

References for anti-IDO1 antibody (ABIN1169195)

Jung, Lee, Chang, Lee, Jeong, Lee, Park, Han, Seo, Lee, Park: "Blockade of indoleamine 2,3-dioxygenase protects mice against lipopolysaccharide-induced endotoxin shock." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 3146-54, (2009) (PubMed).

Yen, Lin, Chen, Huang, Yang, Chang, Lei, Lai: "A novel cancer therapy by skin delivery of indoleamine 2,3-dioxygenase siRNA." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 641-9, (2009) (PubMed).

Cook, Bickerstaff, Wang, Nadasdy, Della Pelle, Colvin, Orosz: "Spontaneous renal allograft acceptance associated with "regulatory" dendritic cells and IDO." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 3103-12, (2008) (PubMed).

Yadav, Burudi, Alirezaei, Flynn, Watry, Lanigan, Fox: "IFN-gamma-induced IDO and WRS expression in microglia is differentially regulated by IL-4." in: Glia, Vol. 55, Issue 13, pp. 1385-96, (2007) (PubMed).

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO (IDO1 Products)

Background: IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

UniProt: P28776

Pathways: Activated T Cell Proliferation