PIK3R1 antibody

Catalog No. ABIN1108652

The Mouse Monoclonal anti-PIK3R1 antibody is suitable to detect PIK3R1 in samples from Human, Mouse and Rat. It has been validated for WB, IF and IP.

Quick Overview for PIK3R1 antibody (ABIN1108652)

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3R1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB6

Product Details anti-PIK3R1 Antibody

Specificity: This antibody reacts with p85alpha, which is regulatory subunit of PI3-kinase on Western blot, Immunoprecipitation and Immunocytochemistry. This antibody recognizes mutant p85alpha protein expressed in E. coli to be possessed in amino acid position '1-79' described below. This antibody does not react with p85beta.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Protein A agarose

Immunogen: Recombinant human p85alpha.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for PIK3R1

Target: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Alternative Name: PIK3R1 / GRB1

Background: Phosphoinositide 3-kinase (PI3K) family of enzymes generate 3-phosphorylated phosphoinositides that act as second messengers downstream of numerous cellular receptors. This family control several cellular responses including cell growth, survival cytoskeletal remodeling and the trafficking of intracellular organelles in many different cell types. There are four subgroups (class IA, IB, II, III) of PI3K with distinct structure and substrate specificity. Class IA PI3K, which function downstream of activated tyrosine kinases, are heterodimers composed of a p110 catalytic subunit (a, b, or o) and a tightly associated regulatory subunit (p85a, p55a, p50a, p85B, or p55y). Each regulatory subunit can interchangeably associate with different catalytic subunits and shows a unique tissue distribution. p85a is the major regulatory subunit of class IA PI3Ks in most types of cells including immune cells.Synonyms: PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha

Gene ID: 5295

NCBI Accession: NP_852556

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effect