PMVK antibody (Middle Region)

Catalog No. ABIN1108679

The Rabbit Polyclonal anti-PMVK antibody is suitable to detect PMVK in samples from Human, Mouse and Rat. It has been validated for WB, IF and IHC (p).

Quick Overview for PMVK antibody (Middle Region) (ABIN1108679)

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PMVK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PMVK Antibody

Binding Specificity: Middle Region

Specificity: This antibody detects PMVK (mid region). No cross reactivity with other proteins.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to a sequence mapping at the middle region of Human PMVK .Remarks: Sequence is different from the related Mouse and Rat sequence by single amino acid.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 0.2 mg Na2HPO4, 0.9 mg NaCl, 0.05 mg Thimerosal, 0.05 mg Sodium Azide, 5 mg BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PMVK

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Alternative Name: PMVK

Background: PMVK (phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence (PTS-1), Ser-Arg-Leu, at the C terminus of the protein.Synonyms: HUMPMKI, PMKI, PMKase, Phosphomevalonate kinase

Gene ID: 10654

NCBI Accession: NP_006547

UniProt: Q15126